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Product Info Summary
| SKU: | EK1738 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). |
Product info
Product Name
Human Glypican-5/GPC5 ELISA Kit PicoKine®
View all Glypican 5 ELISA kits
SKU/Catalog Number
EK1738
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Glypican-5/GPC5 ELISA Kit PicoKine® (96 Tests). Quantitate Human GPC5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Glypican-5/GPC5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1738)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E25-T554
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1738 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1738 is reactive to GPC5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).
Application Guarantee
EK1738 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Glypican 5
Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1738-CAP | Anti-Human GPC5 Pre-coated 96-well strip microplate | 1 |
| EK1738-ST | Human GPC5 Standard | 2 vials, 10 ng/tube |
| EK1738-DA | Human GPC5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.073 | 0.141 | 0.231 | 0.332 | 0.562 | 0.854 | 1.451 | 2.071 |
Data Example Images
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Human Glypican-5/GPC5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Glypican 5, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Glypican-5/GPC5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 60 | 226 | 938 | 62 | 243 | 967 |
| Standard deviation | 2.58 | 11.07 | 50.7 | 4.15 | 16.77 | 72.5 |
| CV (%) | 4.3% | 4.9% | 5.4% | 6.7% | 6.9% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Glypican 5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 72 | 63 | 72 | 66 | 5.35 | 8.1% |
| Sample 2 | 226 | 232 | 264 | 250 | 243 | 15.00 | 6.1% |
| Sample 3 | 938 | 925 | 1033 | 1044 | 985 | 53.83 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For GPC5 (Source: Uniprot.Org, NCBI)
Gene Name
GPC5
Full Name
Glypican-5
Weight
63.707kDa
Superfamily
glypican family
Alternative Names
Glypican-5; Secreted glypican-5; GPC5 GPC5 glypican 5 glypican-5|bA93M14.1|glypican proteoglycan 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GPC5, check out the GPC5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GPC5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Glypican-5/GPC5 ELISA Kit PicoKine® (EK1738)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Human Glypican-5/GPC5 ELISA Kit PicoKine®
Question
Q: Can Glypican 5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-05-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-05-16
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-01-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-01-27
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Y. Zhang
Verified customer
Asked: 2017-11-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-11-02
