Product Info Summary
SKU: | M01922-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Glypican 3 Monoclonal Antibody
View all Glypican 3 Antibodies
SKU/Catalog Number
M01922-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glypican 3 Monoclonal Antibody catalog # M01922-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glypican 3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01922-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACOO-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glypican 3 Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01922-1 is reactive to GPC3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
84640 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01922-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Positive Control
WB: HepG2 cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Glypican 3 expression in HepG2 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GPC3 (Source: Uniprot.org, NCBI)
Gene Name
GPC3
Full Name
Glypican-3
Weight
84640 MW
Superfamily
glypican family
Alternative Names
Inhibitor of nuclear factor kappa-B kinase subunit alpha;I-kappa-B kinase alpha;IKK-A;IKK-alpha;IkBKA;IkappaB kinase;2.7.11.10;Conserved helix-loop-helix ubiquitous kinase;I-kappa-B kinase 1;IKK1;Nuclear factor NF-kappa-B inhibitor kinase alpha;NFKBIKA;Transcription factor 16;TCF-16;CHUK;IKKA, TCF16; GPC3 DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1 glypican 3 glypican-3|glypican proteoglycan 3|heparan sulphate proteoglycan|intestinal protein OCI-5|secreted glypican-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPC3, check out the GPC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glypican 3 Monoclonal Antibody (M01922-1)
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No publications found for M01922-1
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1 Customer Q&As for Anti-Glypican 3 Monoclonal Antibody
Question
We are currently using anti-Glypican 3 Monoclonal antibody M01922-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
M. Krishna
Verified customer
Asked: 2015-08-27
Answer
The anti-Glypican 3 Monoclonal antibody (M01922-1) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-27