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Product Info Summary
| SKU: | A07532 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GPC5/Glypican 5 Antibody
View all Glypican 5 Antibodies
SKU/Catalog Number
A07532
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GPC5/Glypican 5 Antibody (Catalog # A07532). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPC5/Glypican 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07532)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human GPC5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07532 is reactive to GPC5 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
63707 MW
Background of Glypican 5
Cell surface proteoglycan that bears heparan sulfate By similarity.
Veugelers M., Genomics 40:24-30(1997).
Saunders S., Dev. Biol. 190:78-93(1997).
Dunham A., Nature 428:522-528(2004).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07532 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using GPC5 antibody A07532.
Protein Target Info & Infographic
Gene/Protein Information For GPC5 (Source: Uniprot.org, NCBI)
Gene Name
GPC5
Full Name
Glypican-5
Weight
63707 MW
Superfamily
glypican family
Alternative Names
Glypican-5;Secreted glypican-5;GPC5; GPC5 glypican 5 glypican-5|bA93M14.1|glypican proteoglycan 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPC5, check out the GPC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPC5/Glypican 5 Antibody (A07532)
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No publications found for A07532
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-GPC5/Glypican 5 Antibody
Question
Does anti-GPC5/Glypican 5 antibody A07532 work for WB with amygdala?
K. Baker
Verified customer
Asked: 2020-04-24
Answer
According to the expression profile of amygdala, GPC5 is highly expressed in amygdala. So, it is likely that anti-GPC5/Glypican 5 antibody A07532 will work for WB with amygdala.
Boster Scientific Support
Answered: 2020-04-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-GPC5/Glypican 5 antibody A07532. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
I have a question about product A07532, anti-GPC5/Glypican 5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07532 anti-GPC5/Glypican 5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-08
Question
I am interested in to test anti-GPC5/Glypican 5 antibody A07532 on mouse amygdala for research purposes, then I may be interested in using anti-GPC5/Glypican 5 antibody A07532 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
The products we sell, including anti-GPC5/Glypican 5 antibody A07532, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-29
Question
Do you have a BSA free version of anti-GPC5/Glypican 5 antibody A07532 available?
M. Bhatt
Verified customer
Asked: 2018-01-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPC5/Glypican 5 antibody A07532 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-01
Question
We are currently using anti-GPC5/Glypican 5 antibody A07532 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The anti-GPC5/Glypican 5 antibody (A07532) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-07
