Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody

Product Name

Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody

View all Glycogen Synthase Antibodies

SKU/Catalog Number

P03512-3

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody (Catalog # P03512-3). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store Phospho GYS Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Glycogen Synthase 1 phospho S641 GYS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03512-3)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Human Muscle Glycogen Synthase phospho peptide corresponding to a S641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P03512-3 is reactive to GYS1 in Human, Mouse

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

216500 MW

Background of Glycogen Synthase

Anti-Glycogen synthase 1 pS641 is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P03512-3 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:1,000 - 1:5,000
WB: 1:1,000 - 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For GYS1 (Source: Uniprot.org, NCBI)

Alternative Names

muscle glycogen synthase pS641, glycogen synthase pS641, Glycogen, Glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen synthase1, GYS 1, GYS-1, GYS1, Starch synthase muscle GYS1 GSY, GYS glycogen synthase 1 glycogen [starch] synthase, muscle|glycogen synthase 1 (muscle)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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