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Product Info Summary
| SKU: | M01272 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Glutaminase GLS Rabbit Monoclonal Antibody
View all Glutaminase Antibodies
SKU/Catalog Number
M01272
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glutaminase GLS Rabbit Monoclonal Antibody catalog # M01272. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutaminase GLS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01272)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FIA-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glutaminase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01272 is reactive to GLS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
73461 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01272 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: 293T cell
IHC: human brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Glutaminase expression in 293T cell lysate.
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Immunohistochemical analysis of paraffin-embedded human brain, using Glutaminase Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GLS (Source: Uniprot.org, NCBI)
Gene Name
GLS
Full Name
Glutaminase kidney isoform, mitochondrial
Weight
73461 MW
Superfamily
glutaminase family
Alternative Names
Glutaminase kidney isoform, mitochondrial;GLS;3.5.1.2;K-glutaminase;L-glutamine amidohydrolase;GLS;GLS1, KIAA0838; GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG1, KGA, GLS glutaminase glutaminase kidney isoform, mitochondrial|K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLS, check out the GLS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glutaminase GLS Rabbit Monoclonal Antibody (M01272)
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No publications found for M01272
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Glutaminase GLS Rabbit Monoclonal Antibody
Question
Would anti-Glutaminase Rabbit Monoclonal antibody M01272 work for IHC with brain?
V. Miller
Verified customer
Asked: 2019-04-19
Answer
According to the expression profile of brain, GLS is highly expressed in brain. So, it is likely that anti-Glutaminase Rabbit Monoclonal antibody M01272 will work for IHC with brain.
Boster Scientific Support
Answered: 2019-04-19
Question
Do you have a BSA free version of anti-Glutaminase Rabbit Monoclonal antibody M01272 available?
Verified Customer
Verified customer
Asked: 2019-01-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Glutaminase Rabbit Monoclonal antibody M01272 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Glutaminase Rabbit Monoclonal antibody M01272. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-10
Question
Does M01272 anti-Glutaminase Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
You can see on the product datasheet, M01272 anti-Glutaminase Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-26
Question
We are currently using anti-Glutaminase Rabbit Monoclonal antibody M01272 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
The anti-Glutaminase Rabbit Monoclonal antibody (M01272) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-05
Question
Is this M01272 anti-Glutaminase Rabbit Monoclonal antibody reactive to the isotypes of GLS?
S. Li
Verified customer
Asked: 2014-01-13
Answer
The immunogen of M01272 anti-Glutaminase Rabbit Monoclonal antibody is A synthesized peptide derived from human Glutaminase. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-13
