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Product Info Summary
| SKU: | A04992 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-GLU2B PRKCSH Antibody
SKU/Catalog Number
A04992
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GLU2B PRKCSH Antibody (Catalog # A04992). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLU2B PRKCSH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04992)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GLU2B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04992 is reactive to PRKCSH in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
59425 MW
Background of PRKCSH
Regulatory subunit of glucosidase II.
Ophoff R.A., Eur. J. Hum. Genet. 4:321-328(1996).
Pelletier M.F., Glycobiology 10:815-827(2000).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04992 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2, 293 and HUVEC cells, using GLU2B antibody A04992.
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Immunofluorescence analysis of HeLa cells, using GLU2B antibody A04992.
Protein Target Info & Infographic
Gene/Protein Information For PRKCSH (Source: Uniprot.org, NCBI)
Gene Name
PRKCSH
Full Name
Glucosidase 2 subunit beta
Weight
59425 MW
Alternative Names
Glucosidase 2 subunit beta;80K-H protein;Glucosidase II subunit beta;Protein kinase C substrate 60.1 kDa protein heavy chain;PKCSH;PRKCSH;G19P1; PRKCSH AGE-R2, G19P1, GIIB, PCLD, PCLD1, PKCSH, PLD1, VASAP-60 protein kinase C substrate 80K-H glucosidase 2 subunit beta|AGE-binding receptor 2|advanced glycation end-product receptor 2|glucosidase II subunit beta|hepatocystin|protein kinase C substrate 60.1 kDa protein heavy chain|protein kinase C substrate, 80 Kda protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRKCSH, check out the PRKCSH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRKCSH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLU2B PRKCSH Antibody (A04992)
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No publications found for A04992
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-GLU2B PRKCSH Antibody
Question
Can you help my question with product A04992, anti-GLU2B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04992 anti-GLU2B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-28
Question
Does anti-GLU2B antibody A04992 work on canine IF with platelet?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-GLU2B antibody A04992 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GLU2B antibody A04992 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine platelet in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-GLU2B antibody A04992 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-GLU2B antibody (A04992) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-GLU2B antibody A04992. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Do you have a BSA free version of anti-GLU2B antibody A04992 available?
M. Baker
Verified customer
Asked: 2019-10-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GLU2B antibody A04992 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-22
Question
I have attached the WB image, lot number and protocol we used for platelet using anti-GLU2B antibody A04992. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this A04992 anti-GLU2B antibody reactive to the isotypes of PRKCSH?
O. Patel
Verified customer
Asked: 2018-08-07
Answer
The immunogen of A04992 anti-GLU2B antibody is Synthesized peptide derived from internal of human GLU2B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-07
Question
Would anti-GLU2B antibody A04992 work for IF with platelet?
M. Jones
Verified customer
Asked: 2018-07-02
Answer
According to the expression profile of platelet, PRKCSH is highly expressed in platelet. So, it is likely that anti-GLU2B antibody A04992 will work for IF with platelet.
Boster Scientific Support
Answered: 2018-07-02
Question
I am looking for to test anti-GLU2B antibody A04992 on human platelet for research purposes, then I may be interested in using anti-GLU2B antibody A04992 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
The products we sell, including anti-GLU2B antibody A04992, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-18
Question
I was wanting to use your anti-GLU2B antibody for IF for human platelet on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human platelet identification?
D. Roberts
Verified customer
Asked: 2018-04-19
Answer
It shows on the product datasheet, A04992 anti-GLU2B antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-19
Question
I see that the anti-GLU2B antibody A04992 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-02
Question
Does A04992 anti-GLU2B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Wu
Verified customer
Asked: 2017-03-27
Answer
As indicated on the product datasheet, A04992 anti-GLU2B antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-27
Question
Is a blocking peptide available for product anti-GLU2B antibody (A04992)?
T. Jha
Verified customer
Asked: 2015-04-23
Answer
We do provide the blocking peptide for product anti-GLU2B antibody (A04992). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-23
