Product Info Summary
SKU: | P04660 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-Phospho NEK9 (Thr210) Antibody
SKU/Catalog Number
P04660
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho NEK9 (Thr210) Antibody (Catalog# P04660). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho NEK9 (Thr210) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04660)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human NEK9 around the phosphorylation site of Thr210. Immunogen sequence location: 176-225
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P04660 is reactive to NEK9 in Human, Mouse, Rat
Applications
P04660 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
107168 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
![NEK9 primary antibodies P04660 WB testing NEK9 primary antibodies P04660 WB testing](https://www.bosterbio.com/media/catalog/product/N/E/NEK9-primary-antibodies-P04660-WB-testing.jpg)
Click image to see more details
Western blot analysis of lysates from HepG2 cells, using NEK9 (Phospho-Thr210) Antibody. The lane on the right is blocked with the phospho peptide.
![NEK9 primary antibodies P04660 IF testing NEK9 primary antibodies P04660 IF testing](https://www.bosterbio.com/media/catalog/product/N/E/NEK9-primary-antibodies-P04660-IF-testing.jpg)
Click image to see more details
Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For NEK9 (Source: Uniprot.org, NCBI)
Gene Name
NEK9
Full Name
Serine/threonine-protein kinase Nek9
Weight
107168 MW
Superfamily
protein kinase superfamily
Alternative Names
DKFZp434D0935; KIAA1995; MGC138306; MGC16714; NEK8; Nek8NERCC1; Nercc1 kinase; NERCCEC 2.7.11.1; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a)- related kinase 9; NimA-related kinase 8; NIMA-related kinase Nek8; NimA-related protein kinase 9; serine/threonine-protein kinase Nek9 NEK9 APUG, LCCS10, NC, NERCC, NERCC1 NIMA related kinase 9 serine/threonine-protein kinase Nek9|NIMA (never in mitosis gene a)- related kinase 9|nercc1 kinase|nimA-related protein kinase 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEK9, check out the NEK9 Infographic
![NEK9 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho NEK9 (Thr210) Antibody (P04660)
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No publications found for P04660
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho NEK9 (Thr210) Antibody
Question
I was wanting to use your anti-Phospho NEK9 (Thr210) antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
It shows on the product datasheet, P04660 anti-Phospho NEK9 (Thr210) antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-27
Question
We want to test anti-Phospho NEK9 (Thr210) antibody P04660 on rat leukemic t-cell for research purposes, then I may be interested in using anti-Phospho NEK9 (Thr210) antibody P04660 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Walker
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-Phospho NEK9 (Thr210) antibody P04660, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Phospho NEK9 (Thr210) antibody P04660 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The anti-Phospho NEK9 (Thr210) antibody (P04660) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-18
Question
Does anti-Phospho NEK9 (Thr210) antibody P04660 work for WB with leukemic t-cell?
L. Krishna
Verified customer
Asked: 2018-05-08
Answer
According to the expression profile of leukemic t-cell, NEK9 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho NEK9 (Thr210) antibody P04660 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2018-05-08