Anti-Phospho NEK9 (Thr210) Antibody

NEK9 antibody

Boster Bio Anti-Phospho NEK9 (Thr210) Antibody (Catalog# P04660). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P04660
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

Product Name

Anti-Phospho NEK9 (Thr210) Antibody

View all NEK9 Antibodies

SKU/Catalog Number

P04660

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho NEK9 (Thr210) Antibody (Catalog# P04660). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho NEK9 (Thr210) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04660)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human NEK9 around the phosphorylation site of Thr210. Immunogen sequence location: 176-225

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P04660 is reactive to NEK9 in Human, Mouse, Rat

Applications

P04660 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

107168 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For NEK9 (Source: Uniprot.org, NCBI)

Gene Name

NEK9

Full Name

Serine/threonine-protein kinase Nek9

Weight

107168 MW

Superfamily

protein kinase superfamily

Alternative Names

DKFZp434D0935; KIAA1995; MGC138306; MGC16714; NEK8; Nek8NERCC1; Nercc1 kinase; NERCCEC 2.7.11.1; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a)- related kinase 9; NimA-related kinase 8; NIMA-related kinase Nek8; NimA-related protein kinase 9; serine/threonine-protein kinase Nek9 NEK9 APUG, LCCS10, NC, NERCC, NERCC1 NIMA related kinase 9 serine/threonine-protein kinase Nek9|NIMA (never in mitosis gene a)- related kinase 9|nercc1 kinase|nimA-related protein kinase 9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NEK9, check out the NEK9 Infographic

NEK9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NEK9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for P04660

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Phospho NEK9 (Thr210) Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-Phospho NEK9 (Thr210) Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-Phospho NEK9 (Thr210) Antibody

Question

I was wanting to use your anti-Phospho NEK9 (Thr210) antibody for WB for rat leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

It shows on the product datasheet, P04660 anti-Phospho NEK9 (Thr210) antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-27

Question

We want to test anti-Phospho NEK9 (Thr210) antibody P04660 on rat leukemic t-cell for research purposes, then I may be interested in using anti-Phospho NEK9 (Thr210) antibody P04660 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Walker

Verified customer

Asked: 2019-10-25

Answer

The products we sell, including anti-Phospho NEK9 (Thr210) antibody P04660, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-25

Question

We are currently using anti-Phospho NEK9 (Thr210) antibody P04660 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

The anti-Phospho NEK9 (Thr210) antibody (P04660) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-18

Question

Does anti-Phospho NEK9 (Thr210) antibody P04660 work for WB with leukemic t-cell?

L. Krishna

Verified customer

Asked: 2018-05-08

Answer

According to the expression profile of leukemic t-cell, NEK9 is highly expressed in leukemic t-cell. So, it is likely that anti-Phospho NEK9 (Thr210) antibody P04660 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2018-05-08

Order DetailsPrice
P04660

100 μL

$335

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
P04660
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.