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Product Info Summary
| SKU: | M01703 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GLO1 Monoclonal Antibody
View all Glyoxalase I Antibodies
SKU/Catalog Number
M01703
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GLO1 Monoclonal Antibody catalog # M01703. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLO1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01703)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFDI-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GLO1 Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01703 is reactive to GLO1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
v
Calculated molecular weight
210715 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01703 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
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Western blot analysis of GLO1 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GLO1 (Source: Uniprot.org, NCBI)
Gene Name
GLO1
Full Name
Lactoylglutathione lyase
Weight
210715 MW
Superfamily
glyoxalase I family
Alternative Names
Myosin light chain kinase, smooth muscle;MLCK;smMLCK;2.7.11.18;Kinase-related protein;KRP;Telokin;Myosin light chain kinase, smooth muscle, deglutamylated form;MYLK;MLCK, MLCK1, MYLK1; GLO1 GLOD1, GLYI, HEL-S-74 glyoxalase I lactoylglutathione lyase|S-D-lactoylglutathione methylglyoxal lyase|aldoketomutase|epididymis secretory protein Li 74|glx I|glyoxalase domain containing 1|ketone-aldehyde mutase|lactoyl glutathione lyase|methylglyoxalase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLO1, check out the GLO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLO1 Monoclonal Antibody (M01703)
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No publications found for M01703
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Customer Q&As
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6 Customer Q&As for Anti-GLO1 Monoclonal Antibody
Question
I have a question about product M01703, anti-GLO1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01703 anti-GLO1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-17
Question
Does anti-GLO1 Monoclonal antibody M01703 work for WB with prostate gland?
M. Mangal
Verified customer
Asked: 2019-12-05
Answer
According to the expression profile of prostate gland, GLO1 is highly expressed in prostate gland. So, it is likely that anti-GLO1 Monoclonal antibody M01703 will work for WB with prostate gland.
Boster Scientific Support
Answered: 2019-12-05
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostate gland using anti-GLO1 Monoclonal antibody M01703. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-03
Question
you antibody to test anti-GLO1 Monoclonal antibody M01703 on rat prostate gland for research purposes, then I may be interested in using anti-GLO1 Monoclonal antibody M01703 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The products we sell, including anti-GLO1 Monoclonal antibody M01703, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-07
Question
Would M01703 anti-GLO1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-12
Answer
As indicated on the product datasheet, M01703 anti-GLO1 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-12
Question
Please see the WB image, lot number and protocol we used for prostate gland using anti-GLO1 Monoclonal antibody M01703. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-25
