Product Info Summary
SKU: | PA1766 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®
SKU/Catalog Number
PA1766
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcriptional activator GLI3 Gli3 Antibody catalog # PA1766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1766)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1766 is reactive to GLI3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
169863 MW
Background of GLI-3
GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1766 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Testis Tissue, A549 Cell, SW620 Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-Gli3 antibody, PA1766, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: SW620 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For GLI3 (Source: Uniprot.org, NCBI)
Gene Name
GLI3
Full Name
Transcriptional activator GLI3
Weight
169863 MW
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
Transcriptional activator GLI3;GLI3 form of 190 kDa;GLI3-190;GLI3 full length protein;GLI3FL;Transcriptional repressor GLI3R;GLI3 C-terminally truncated form;GLI3 form of 83 kDa;GLI3-83;GLI3; GLI3 ACLS, GCPS-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, GLI3 GLI family zinc finger 3 transcriptional activator GLI3|GLI-Kruppel family member GLI3|glioma-associated oncogene family zinc finger 3|oncogene GLI3|zinc finger protein GLI3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLI3, check out the GLI3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband® (PA1766)
Hello CJ!
PA1766 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Kumari S, Chaurasia Sn, Kumar K, Dash D. Thromb Res. 2014 Dec;134(6):1311-5. Doi: 10.1016/J.Thromres.2014.09.022. Epub 2014 Sep 28. Anti-Apoptotic Role Of Sonic Hedgehog On Blood Platelets.
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Customer Q&As
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5 Customer Q&As for Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®
Question
I was wanting to use your anti-Gli3 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
It shows on the product datasheet, PA1766 anti-Gli3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-04
Question
Will anti-Gli3 antibody PA1766 work on goat WB with cerebellum?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
Our lab technicians have not validated anti-Gli3 antibody PA1766 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Gli3 antibody PA1766 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-21
Question
We are currently using anti-Gli3 antibody PA1766 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
N. Thomas
Verified customer
Asked: 2014-10-29
Answer
The anti-Gli3 antibody (PA1766) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-29
Question
Is this PA1766 anti-Gli3 antibody reactive to the isotypes of GLI3?
J. Carter
Verified customer
Asked: 2014-05-06
Answer
The immunogen of PA1766 anti-Gli3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3(40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-05-06
Question
Is a blocking peptide available for product anti-Gli3 antibody (PA1766)?
E. Williams
Verified customer
Asked: 2013-04-08
Answer
We do provide the blocking peptide for product anti-Gli3 antibody (PA1766). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-04-08