Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

GLI-3 antibody

Boster Bio Anti-Transcriptional activator GLI3 Gli3 Antibody catalog # PA1766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PA1766
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

View all GLI-3 Antibodies

SKU/Catalog Number

PA1766

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Transcriptional activator GLI3 Gli3 Antibody catalog # PA1766. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1766)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1766 is reactive to GLI3 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

33 kDa

Calculated molecular weight

169863 MW

Background of GLI-3

GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1766 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Testis Tissue, A549 Cell, SW620 Cell,

Validation Images & Assay Conditions

Gene/Protein Information For GLI3 (Source: Uniprot.org, NCBI)

Gene Name

GLI3

Full Name

Transcriptional activator GLI3

Weight

169863 MW

Superfamily

GLI C2H2-type zinc-finger protein family

Alternative Names

Transcriptional activator GLI3;GLI3 form of 190 kDa;GLI3-190;GLI3 full length protein;GLI3FL;Transcriptional repressor GLI3R;GLI3 C-terminally truncated form;GLI3 form of 83 kDa;GLI3-83;GLI3; GLI3 ACLS, GCPS-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, GLI3 GLI family zinc finger 3 transcriptional activator GLI3|GLI-Kruppel family member GLI3|glioma-associated oncogene family zinc finger 3|oncogene GLI3|zinc finger protein GLI3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GLI3, check out the GLI3 Infographic

GLI3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GLI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1766 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Kumari S, Chaurasia Sn, Kumar K, Dash D. Thromb Res. 2014 Dec;134(6):1311-5. Doi: 10.1016/J.Thromres.2014.09.022. Epub 2014 Sep 28. Anti-Apoptotic Role Of Sonic Hedgehog On Blood Platelets.

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5 Customer Q&As for Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

Question

I was wanting to use your anti-Gli3 antibody for WB for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

It shows on the product datasheet, PA1766 anti-Gli3 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-04

Question

Will anti-Gli3 antibody PA1766 work on goat WB with cerebellum?

Verified Customer

Verified customer

Asked: 2018-09-21

Answer

Our lab technicians have not validated anti-Gli3 antibody PA1766 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Gli3 antibody PA1766 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cerebellum in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-09-21

Question

We are currently using anti-Gli3 antibody PA1766 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

N. Thomas

Verified customer

Asked: 2014-10-29

Answer

The anti-Gli3 antibody (PA1766) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-10-29

Question

Is this PA1766 anti-Gli3 antibody reactive to the isotypes of GLI3?

J. Carter

Verified customer

Asked: 2014-05-06

Answer

The immunogen of PA1766 anti-Gli3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3(40-57aa SNEDESPGQTYHRERRNA), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-05-06

Question

Is a blocking peptide available for product anti-Gli3 antibody (PA1766)?

E. Williams

Verified customer

Asked: 2013-04-08

Answer

We do provide the blocking peptide for product anti-Gli3 antibody (PA1766). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-04-08

Order DetailsPrice
PA1766

100μg

$370
PA1766-10ug

10μg sample (liquid)

$99
PA1766-Biotin

100 μg Biotin conjugated

$570
PA1766-Cy3

100 μg Cy3 conjugated

$570
PA1766-Dylight488

100 μg Dylight488 conjugated

$570
PA1766-Dylight550

100 μg Dylight550 conjugated

$570
PA1766-Dylight594

100 μg Dylight594 conjugated

$570
PA1766-FITC

100 μg FITC conjugated

$570
PA1766-HRP

100 μg HRP conjugated

$570
PA1766-APC

100 μg APC conjugated

$670
PA1766-PE

100 μg PE conjugated

$670
PA1766-iFluor647

100 μg iFluor647 conjugated

$670
PA1766-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1766
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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