Click image to see more details
Product Info Summary
| SKU: | A01647-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GLCNE GNE Antibody
View all N-Acetylmannosamine Kinase/GNE Antibodies
SKU/Catalog Number
A01647-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GLCNE GNE Antibody catalog # A01647-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GLCNE GNE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01647-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GNE. AA range:592-641
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01647-1 is reactive to GNE in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
79275 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01647-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 3T3 cells using GLCNE Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GNE (Source: Uniprot.org, NCBI)
Gene Name
GNE
Full Name
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
Weight
79275 MW
Alternative Names
Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase;UDP-GlcNAc-2-epimerase/ManAc kinase;UDP-N-acetylglucosamine 2-epimerase (hydrolyzing);3.2.1.183;UDP-GlcNAc-2-epimerase;Uridine diphosphate-N-acetylglucosamine-2-epimerase;N-acetylmannosamine kinase;2.7.1.60;ManAc kinase;GNE;GLCNE; GNE DMRV, GLCNE, IBM2, NM, Uae1 glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase|N-acylmannosamine kinase|UDP-GlcNAc-2-epimerase/ManAc kinase|UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNE, check out the GNE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLCNE GNE Antibody (A01647-1)
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No publications found for A01647-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-GLCNE GNE Antibody
Question
Is a blocking peptide available for product anti-GLCNE antibody (A01647-1)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
We do provide the blocking peptide for product anti-GLCNE antibody (A01647-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-01
Question
See attached the WB image, lot number and protocol we used for small intestine using anti-GLCNE antibody A01647-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
you antibody to test anti-GLCNE antibody A01647-1 on mouse small intestine for research purposes, then I may be interested in using anti-GLCNE antibody A01647-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The products we sell, including anti-GLCNE antibody A01647-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-20
Question
Will A01647-1 anti-GLCNE antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Edwards
Verified customer
Asked: 2020-01-06
Answer
You can see on the product datasheet, A01647-1 anti-GLCNE antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-06
Question
Does anti-GLCNE antibody A01647-1 work on bovine WB with hippocampus tongue?
Verified Customer
Verified customer
Asked: 2018-01-17
Answer
Our lab technicians have not tested anti-GLCNE antibody A01647-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-GLCNE antibody A01647-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine hippocampus tongue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-17
Question
We are currently using anti-GLCNE antibody A01647-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
D. Li
Verified customer
Asked: 2015-04-09
Answer
The anti-GLCNE antibody (A01647-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-09
Question
I see that the anti-GLCNE antibody A01647-1 works with WB, what is the protocol used to produce the result images on the product page?
T. Patel
Verified customer
Asked: 2015-01-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-01-19
