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Product Info Summary
| SKU: | A02140 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GIT1 Antibody
SKU/Catalog Number
A02140
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GIT1 Antibody (Catalog # A02140). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GIT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02140)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human GIT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02140 is reactive to GIT1 in Human
Reconstitution
Calculated molecular weight
84341 MW
Background of GIT1
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
Premont R.T., J. Biol. Chem. 275:22373-22380(2000).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Premont R.T., Proc. Natl. Acad. Sci. U.S.A. 95:14082-14087(1998).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02140 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using GIT1 antibody A02140.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using GIT1 antibody A02140.
Protein Target Info & Infographic
Gene/Protein Information For GIT1 (Source: Uniprot.org, NCBI)
Gene Name
GIT1
Full Name
ARF GTPase-activating protein GIT1
Weight
84341 MW
Alternative Names
ARF GTPase-activating protein GIT1;ARF GAP GIT1;Cool-associated and tyrosine-phosphorylated protein 1;CAT-1;CAT1;G protein-coupled receptor kinase-interactor 1;GRK-interacting protein 1;GIT1; GIT1 GIT ArfGAP 1 ARF GTPase-activating protein GIT1|ARF GAP GIT1|CAT-1|CAT1|G protein-coupled receptor kinase interacting ArfGAP 1|G protein-coupled receptor kinase-interactor 1|GRK-interacting protein 1|cool-associated and tyrosine-phosphorylated protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GIT1, check out the GIT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GIT1 Antibody (A02140)
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