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Product Info Summary
| SKU: | A01351 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-eIF4G (Ab-1231) EIF4G1 Antibody
SKU/Catalog Number
A01351
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-eIF4G (Ab-1231) EIF4G1 Antibody (Catalog # A01351). Tested in WB, IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eIF4G (Ab-1231) EIF4G1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01351)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1229~1233 (P-V-S-P-L) derived from Human eIF4G.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01351 is reactive to EIF4G1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
53365 MW
Background of EIF4G1
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis
De Gregorio, E. et al. (1998) RNA 4, 828-836.
Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
Gradi, A. et al. (1998) Mol Cell Biol 18, 334-42.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01351 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of EIF4G1 using anti-EIF4G1 antibody (A01351).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4G1 antigen affinity purified polyclonal antibody (Catalog # A01351) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for EIF4G1.
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Immunofluorescence staining of methanol-fixed Hela cells using eIF4G (Ab-1231) Antibody #A01351.
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Figure 2. IHC analysis of EIF4G1 using anti-EIF4G1 antibody (A01351).
EIF4G1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EIF4G1 Antibody (A01351) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For EIF4G1 (Source: Uniprot.org, NCBI)
Gene Name
EIF4G1
Full Name
Eukaryotic translation initiation factor 4 gamma 1
Weight
53365 MW
Superfamily
eukaryotic initiation factor 4G family
Alternative Names
M-phase inducer phosphatase 3;3.1.3.48;Dual specificity phosphatase Cdc25C;CDC25C; EIF4G1 EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18 eukaryotic translation initiation factor 4 gamma 1 eukaryotic translation initiation factor 4 gamma 1|EIF4-gamma|eIF-4-gamma 1|eucaryotic translation initiation factor 4G
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4G1, check out the EIF4G1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4G1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eIF4G (Ab-1231) EIF4G1 Antibody (A01351)
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