Product Info Summary
SKU: | A03161-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-GGH Antibody Picoband®
View all gamma-glutamyl hydrolase Antibodies
SKU/Catalog Number
A03161-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GGH Antibody Picoband® catalog # A03161-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GGH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03161-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GGH recombinant protein (Position: R25-D318).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03161-2 is reactive to GGH in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
65411 MW
Background of gamma-glutamyl hydrolase
Gamma-glutamyl hydrolase is encoded by theGGHgene. This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03161-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human HepG2 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GGH using anti-GGH antibody (A03161-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGH antigen affinity purified polyclonal antibody (Catalog # A03161-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGH at approximately 36 kDa. The expected band size for GGH is at 36 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GGH (Source: Uniprot.org, NCBI)
Gene Name
GGH
Full Name
Gamma-glutamyl hydrolase
Weight
65411 MW
Superfamily
peptidase C26 family
Alternative Names
IFNGR2 protein; Interferon gamma receptor 2; IFNGR2; mCG_11456 GGH GH gamma-glutamyl hydrolase gamma-glutamyl hydrolase|gamma-Glu-X carboxypeptidase|gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GGH, check out the GGH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GGH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GGH Antibody Picoband® (A03161-2)
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