Anti-GGA-1 Antibody

Product Name

Anti-GGA-1 Antibody

View all GGA1 Antibodies

SKU/Catalog Number

A02501

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-GGA-1 Antibody (Catalog # A02501). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-GGA-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02501)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02501 is reactive to GGA1 in Human

Applications

A02501 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

61762 MW

Background of GGA1

GGA1 (also known as ADP-ribosylation factor binding protein, Golgi-localized, gamma ear-containing, ARF-binding protein 1, Gamma-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IP: 1:100
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Western blot analysis of GGA1 expression in HEK293 cells over-expressing the recombinant protein (arrowhead). GGA1 at 100KD was detected using rabbit anti-GGA1 Antigen Affinity purified polyclonal antibody (Catalog # A02501) at 1:1350. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For GGA1 (Source: Uniprot.org, NCBI)

Alternative Names

ADP-ribosylation factor binding protein 1; ADP-ribosylation factor-binding protein GGA1; gamma adaptin ear containing, ARF binding protein 1; gamma-adaptin related protein 1; Gamma-adaptin-related protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1 GGA1 golgi associated, gamma adaptin ear containing, ARF binding protein 1 ADP-ribosylation factor-binding protein GGA1|ADP-ribosylation factor binding protein 1|gamma-adaptin related protein 1|golgi-localized, gamma ear-containing, ARF-binding protein 1

For more info on GGA1, check out the GGA1 Infographic

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In this infographic, you will see the following information for GGA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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