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Product Info Summary
| SKU: | M04560-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Gephyrin Rabbit Monoclonal Antibody
View all Gephyrin/GPHN Antibodies
SKU/Catalog Number
M04560-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Gephyrin Rabbit Monoclonal Antibody catalog # M04560-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gephyrin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04560-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
18G74
Isotype
IgG
Immunogen
A synthesized peptide derived from human Gephyrin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04560-3 is reactive to GPHN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
93 kDa
Calculated molecular weight
46757 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04560-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human 293T whole cell, human U20S whole cell, human HepG2 whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Gephyrin using anti-Gephyrin antibody (M04560-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U20S whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gephyrin antigen affinity purified monoclonal antibody (Catalog # M04560-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gephyrin at approximately 93 kDa. The expected band size for Gephyrin is at 80 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GPHN (Source: Uniprot.org, NCBI)
Gene Name
GPHN
Full Name
Gephyrin
Weight
46757 MW
Alternative Names
G1/S-specific cyclin-E2;CCNE2; GPHN GEPH, GPH, GPHRYN, HKPX1, MOCODC gephyrin gephyrin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPHN, check out the GPHN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPHN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gephyrin Rabbit Monoclonal Antibody (M04560-3)
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