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Product Info Summary
| SKU: | M04553 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IP, IF, WB |
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Product info
Product Name
Anti-CENP-E Monoclonal Antibody
SKU/Catalog Number
M04553
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-CENP-E Monoclonal Antibody (Catalog # M04553). Tested in IF, IP, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CENP-E Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04553)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 1H12 IgG1
Isotype
IgG1
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04553 is reactive to CENPE in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
316415 MW
Background of CENPE
CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase. CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04553 is guaranteed for IP, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:20,000
IF Microscopy: 1:500 - 1:2,000
IP: 1:200
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Mouse anti-CENPE monoclonal antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.
Protein Target Info & Infographic
Gene/Protein Information For CENPE (Source: Uniprot.org, NCBI)
Gene Name
CENPE
Full Name
Centromere-associated protein E
Weight
316415 MW
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
CENP-E, Centromere-associated protein E, Centromeric protein E, KIF10, Kinesin-7, Kinesin family member 10, Kinesin-related protein CENPE CENPE CENP-E, KIF10, MCPH13, PPP1R61 centromere protein E centromere-associated protein E|Centromere auto E (312kD)|centromere protein E, 312kDa|kinesin family member 10|kinesin-7|kinesin-related protein CENPE|protein phosphatase 1, regulatory subunit 61
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CENPE, check out the CENPE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CENPE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CENP-E Monoclonal Antibody (M04553)
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