Gephyrin Antibodies

Gephyrin (GPHN)

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It's supposed to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules.

Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated.

Gene Information

Human
Gene Name:	GPHN
Uniprot:	Q9NQX3
Entrez:	10243

Family

Belongs to:
No superfamily

Alternative Names

GEPH; Gephyrin; GPH; GPHN; GPHRYN; KIAA1385GPHGEPH

Molecular Weight

Mass (kDA):
79.748 kDA

Genomic Context

Human
Location:	14q23.3-q24.1
Sequence:	14; NC_000014.9 (66507934..67181805)

Subcellular Localizations

Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, dendrite. Cell junction, synapse, postsynaptic density. Cytoplasmic face of glycinergic postsynaptic membranes.

Diseases

• Hyperexplexia
• Muscle Hypertonia
• Combined Molybdoflavoprotein Enzyme Deficiency
• Nervous System Disorder
• Leukemia
• Mutation, Out-of-frame
• Apnea
• Chromosomal Translocation
• Paraplegia
• Leukemogenesis
• Spastic Paraplegia

• Neoplasms, Experimental
• Trauma, Nervous System
• Dyskinetic Syndrome
• Myeloid Leukemia
• Dyskinesia, Drug-induced
• Chromosome Breakage

Interacting Proteins

• DYNLL1

Pathways

• Startle Response
• Localization
• Chromosome Localization
• Error-prone Dna Repair
• Dna Repair
• Synaptic Transmission

• Reflex

PTMs

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GPHN

Best Uses Of The GPHN Marker From Boster Bio

The Boster Bio company was founded in 1993. Their trademark products are ELISAs as well as antibodies. However, they have recently begun to offer PCR-related molecular biology products. Boster Bio offers many services to scientists including technical assistance, 24 hour customer support, and antibodies. Here are some of the best uses of the GPHN marker. This marker is an excellent tool for gene expression analysis.

Anti-Gephyrin

Boster Bio's Anti-Gephyrin antibody, which targets gephyrin specifically, has been rediscovered by the laboratory. This protein is found throughout a variety of neuronal cells including hippocampal nerve cells. Gephyrin is also found in other neuronal types such as vGAT, EGFP, and others. We will now discuss its potential applications in neuroscience.

Gephyrin can alter the lateral mobility at synapses of GABAARs. Gephyrin impairment causes the lateral dispersion of synaptic GABAARs and their depletion. However, it is not clear what role gephyrin plays in the expression and maintenance of iLTP. It is currently not clear whether gephyrin affects brain excitability.

We also tested the immunoreactivity to gephyrin, vGAT, and mAb7a using mAb3B11 and mAb3B11 antibody. These antibodies are used for staining intracellular proteins, such as gephyrin. These antibodies are both highly effective at detecting gephyrin as well as vGAT. You can search online to find the boster antibody that you are looking. You can buy the boster bioantibody in many different dilutions.

Boster scientists will be invited to submit their species or application results. They are also eligible for product credits. All Boster Bio scientists can apply for the Boster Bio product credit. There are currently no limits on how many times scientists can submit their results to Boster Bio. Send your results to Boster Bio today! It's simple to do, and you could receive the rewards that you've been waiting for!

Gephyrin concentrations at synapses are affected by NMDA stimulation. NMDA also decreases extrasynaptic gephyrin fluorescence, while it did not affect synaptic gephyrin intensity. Gephyrin gets redistributed on a longer scale from extrasynaptic compartments into synaptic compartments.

Gephyrin interactions are possible with several GABAAR subunits. Boster Bio is focused on the gephyrin-GABAAR interactions as a target for drug development. It is also effective in preventing the accumulation of NMDA-induced GABAARs at synapses. Anti-Gephyrin, a new drug, is designed to target this protein. It is now approved by FDA to treat epilepsy.

Membrane staining improves protein transfer efficiency

The new Picokine(tm) platform from Boster Bio further enhances ELISA sensitivity to the picogram level. It has been tested for a variety sample and is available on request. Picoband secondary antibodies are made from polymers and save 30 minutes of IHC. Picoband was created by Boster Bio, who has extensive knowledge of immunogen design. Sanbio is the technical support distributor for BeNeLux.

The membrane's pore sizes play a crucial role in determining protein transfer efficiency. A 0.2 mm membrane is better for detecting low-molecular-weight proteins than one with a molecular weight greater than 20 kD. High-molecular-weight proteins, such as CerP, will bind to the membrane much more efficiently than proteins with lower molecular weights.

To optimize the antibody re-probing capacity of PVDF and NC membranes, a series of experiments were performed on both types of membranes. First, we incubated protein samples with different molecularweights on PVDF membranes. ECL reagents allowed visualization of the proteins on both membranes. The membranes were then reprobed using BSA, or other antibodies. The PVDF membrane's re-probed potential was significantly higher than that for NC membrane. In three cases, the PVDF membrane displayed more advantages than NC membrane.

PVDF membranes require four-fold less serum proteins than the NC membrane to stain with the identical antibodies. The membrane-staining efficiency was improved from 60 to 90 minutes. IgG and A2M antibodies only required 1.5 mg serum proteins. The intensity of antibody staining increased by 1.9 to sixfold. Compared to NC membrane, CerP staining took just 60 minutes to transfer to the PVDF membrane.

Highlighting genes of particular interest by subset of GPHN Marker

Although exonic GPHN DNA deletions were rare in the general populace, a study involving 27 019 SAGE control subjects revealed three cases of exonic GPHN DNA deletions. This finding may reflect variable GPHN expression or false positive calls, as there were no DNA samples available from the two control individuals. Despite this, this study provides important insights into how this deletion works.

Gephyrin encodes a scaffolding-protein found in neuronal postsynaptic cell membrane. It is involved in localization of GABA and glycine receptors at inhibitory synapses. Gephyrin appears to interact with a number of synaptic molecules, including neurexins (collybistin) and neurexins (neurexin). It was previously associated with temporal lobe epilepsy. This is due to the abnormal splicing GPHN mRNA.