Product Info Summary
SKU: | PA1465 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-GDNF Antibody Picoband®
SKU/Catalog Number
PA1465
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GDNF Antibody catalog # PA1465. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1465)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GDNF, different from the related mouse sequence by three amino acids and rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1465 is reactive to GDNF in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
23720 MW
Background of GDNF
GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL). The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1465 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Positive Control
IHC: Rat Brain Tissue, Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-GDNF antibody, PA1465, Western blotting
Lane 1: Recombinant Human GDNF Protein 10ng
Lane 2: Recombinant Human GDNF Protein 5ng
Lane 3: Recombinant Human GDNF Protein 2.5ng
Lane 4: Recombinant Human GDNF Protein 1.25ng
Click image to see more details
Anti-GDNF antibody, PA1465, IHC(P)
IHC(P): Rat Brain Tissue
Click image to see more details
Anti-GDNF antibody, PA1465, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For GDNF (Source: Uniprot.org, NCBI)
Gene Name
GDNF
Full Name
Glial cell line-derived neurotrophic factor
Weight
23720 MW
Superfamily
TGF-beta family
Alternative Names
Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF; GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GDNF, check out the GDNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GDNF Antibody Picoband® (PA1465)
Hello CJ!
PA1465 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction
The interplay of BDNF-TrkB with NMDA receptor in propofol-induced cognition dysfunction
Culture bovine prospermatogonia with 2i medium
Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells
Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction
Li R, Xia W, Zhang Z, Wu K. Plos One. 2011;6(6):E21663. Doi: 10.1371/Journal.Pone.0021663. Epub 2011 Jun 27. S100B Protein, Brain-Derived Neurotrophic Factor, And Glial Cell Line-Derived Neurotrophic Factor In Human Milk.
Zhang X, Shi K, Li Y, Zhang H, Hao J. Reprod Toxicol. 2014 Jun;45:87-93. Doi: 10.1016/J.Reprotox.2014.01.009. Epub 2014 Feb 4. Lipopolysaccharide Inhibits The Self-Renewal Of Spermatogonial Stem Cells In Vitro Via Downregulation Of Gdnf Expression...
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-GDNF Antibody Picoband®
Question
Is PA1465 suitable for ICC?
Verified customer
Asked: 2020-03-10
Answer
The Anti-GDNF Antibody PA1465 is not suitable for Immunocytochemistry.
Boster Scientific Support
Answered: 2020-03-10
Question
Is PA1465 able to detect preGDNF and mature GDNF?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
PA1465 is only able to detect mature GDNF
Boster Scientific Support
Answered: 2019-10-16
Question
Can PA1465 be used to detect GDNF in human testicular tissue?
Verified customer
Asked: 2019-03-26
Answer
Test result of the Anti-GDNF Antibody (PA1465) in human testicular tissue is not good. We do not recommend using PA1465 to test human testicular tissues.
Boster Scientific Support
Answered: 2019-04-01