Product Info Summary
SKU: | FEK0935 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse GDNF PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0935
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse GDNF PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse GDNF PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0935)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S78-I211
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0935 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0935 is reactive to Gdnf in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0935 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GDNF
Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
Kit Components
Catalog number | Description | Quantity |
---|---|---|
FEK0935-CAP | 96-well plate precoated with anti-Mouse GDNF antibody | 1 |
EK0935-ST | lyophilized recombinant Mouse GDNF standard | 10 ng/tube |
FEK0935-DA | Biotinylated anti-Mouse GDNF detection antibody (50x) | 100 ul |
- | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
AR1106-1 | Sample diluent | 30 ml |
AR1106-2 | Antibody diluent | 12 ml |
- | Avidin-Biotin-Peroxidase Diluent | 12 ml |
AR1106-5 | Wash Buffer (25x) | 20 ml |
AR1104 | Color Developing Reagent (TMB) | 10 ml |
AR1105 | Stop Solution | 10 ml |
PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.089 | 0.141 | 0.264 | 0.521 | 0.952 | 1.726 | 2.466 |
Data Example Images
Click image to see more details
Mouse GDNF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse GDNF PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 101 | 227 | 789 | 101 | 217 | 855 |
Standard deviation | 7.57 | 9.3 | 57.53 | 7.67 | 9.11 | 66.69 |
CV (%) | 7.5% | 4.1% | 7% | 7.6% | 4.2% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GDNF in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 101 | 101 | 104 | 97 | 100 | 2.48 | 2.4% |
Sample 2 | 227 | 204 | 214 | 227 | 218 | 9.66 | 4.4% |
Sample 3 | 789 | 928 | 792 | 891 | 850 | 60.93 | 7.1% |
Protein Target Info & Infographic
Gene/Protein Information For Gdnf (Source: Uniprot.Org, NCBI)
Uniprot ID
P48540
Gene ID
2668
Gene Name
Gdnf
Full Name
Glial cell line-derived neurotrophic factor
Weight
23662 MW
Superfamily
TGF-beta family
Alternative Names
Glial cell line-derived neurotrophic factor;mGDNF;Astrocyte-derived trophic factor;ATF;Gdnf; GDNF ATF, ATF1, ATF2, HFB1-GDNF, HSCR3 glial cell derived neurotrophic factor glial cell line-derived neurotrophic factor|astrocyte-derived trophic factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Gdnf, check out the Gdnf Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Gdnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse GDNF PicoKine® Quick ELISA Kit (FEK0935)
Hello CJ!
FEK0935 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury
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