Click image to see more details
Product Info Summary
| SKU: | M01172 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01172
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody catalog # M01172. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01172)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACAA-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCN2 Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01172 is reactive to Eif2ak4 in Human
Applications
M01172 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
190 kDa
Calculated molecular weight
30773 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
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Western blot analysis of GCN2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For Eif2ak4 (Source: Uniprot.org, NCBI)
Gene Name
Eif2ak4
Full Name
eIF-2-alpha kinase GCN2
Weight
30773 MW
Superfamily
protein kinase superfamily
Alternative Names
EIF2AK4; eukaryotic translation initiation factor 2 alpha kinase 4; eukaryotic translation initiation factor 2-alpha kinase 4; GCN2; GCN2GCN2 eIF2alpha kinase; GCN2-like protein; KIAA1338EC 2.7.11.1 Eif2ak4|2610011M03, GCN, GCN2, MGCN2|eukaryotic translation initiation factor 2 alpha kinase 4|eIF-2-alpha kinase GCN2|GCN2 eIF2alpha kinase|GCN2-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Eif2ak4, check out the Eif2ak4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Eif2ak4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody (M01172)
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No publications found for M01172
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody
Question
Can you help my question with product M01172, anti-GCN2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01172 anti-GCN2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-09
Question
Does anti-GCN2 Monoclonal antibody M01172 work for WB with brain?
V. Anderson
Verified customer
Asked: 2020-04-07
Answer
According to the expression profile of brain, EIF2AK4 is highly expressed in brain. So, it is likely that anti-GCN2 Monoclonal antibody M01172 will work for WB with brain.
Boster Scientific Support
Answered: 2020-04-07
Question
Our team were satisfied with the WB result of your anti-GCN2 Monoclonal antibody. However we have observed positive staining in melanoma testis cytoplasm. using this antibody. Is that expected? Could you tell me where is EIF2AK4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
From what I have seen in literature, melanoma testis does express EIF2AK4. Generally EIF2AK4 expresses in cytoplasm. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-01
Question
We are currently using anti-GCN2 Monoclonal antibody M01172 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
The anti-GCN2 Monoclonal antibody (M01172) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-26
Question
Our lab used your anti-GCN2 Monoclonal antibody for WB on muscle uterus a few years ago. I am using human, and We intend to use the antibody for IF next. I was wanting to use examining muscle uterus as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
I have checked the website and datasheets of our anti-GCN2 Monoclonal antibody and I see that M01172 has been validated on human in both WB and IF. Thus M01172 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-26
Question
I see that the anti-GCN2 Monoclonal antibody M01172 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-24
Question
I would like to test anti-GCN2 Monoclonal antibody M01172 on human brain for research purposes, then I may be interested in using anti-GCN2 Monoclonal antibody M01172 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
The products we sell, including anti-GCN2 Monoclonal antibody M01172, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-06
Question
We have seen staining in human adenohypophysis. Do you have any suggestions? Is anti-GCN2 Monoclonal antibody supposed to stain adenohypophysis positively?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
From what I have seen in literature adenohypophysis does express EIF2AK4. From what I have seen in Uniprot.org, EIF2AK4 is expressed in adenohypophysis, muscle uterus, brain, melanoma testis, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EIF2AK4 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Melanoma, and Testis, Pubmed ID: 17974005
Muscle, and Uterus, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-20
Question
What is the sample target on Western blot analysis in your product image for M01172?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-GCN2 EIF2AK4 Rabbit Monoclonal Antibody (M01172) expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
Does M01172 anti-GCN2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
As indicated on the product datasheet, M01172 anti-GCN2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
I am interested in using your anti-GCN2 Monoclonal antibody for eif2alpha phosphorylation in response to endoplasmic reticulum stress studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Thank you for your inquiry. This M01172 anti-GCN2 Monoclonal antibody is tested on hela cell lysate, human tonsil tissue, a549 cells. It is guaranteed to work for Flow Cytometry, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-25
Question
Please see the WB image, lot number and protocol we used for brain using anti-GCN2 Monoclonal antibody M01172. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GCN2 Monoclonal antibody M01172. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-28
Question
Is a blocking peptide available for product anti-GCN2 Monoclonal antibody (M01172)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We do provide the blocking peptide for product anti-GCN2 Monoclonal antibody (M01172). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-22
Question
Is this M01172 anti-GCN2 Monoclonal antibody reactive to the isotypes of EIF2AK4?
A. Walker
Verified customer
Asked: 2017-07-14
Answer
The immunogen of M01172 anti-GCN2 Monoclonal antibody is A synthesized peptide derived from human GCN2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-14
Question
I was wanting to use your anti-GCN2 Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
A. Patel
Verified customer
Asked: 2017-04-28
Answer
It shows on the product datasheet, M01172 anti-GCN2 Monoclonal antibody has been validated for Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-28
Question
Do you have a BSA free version of anti-GCN2 Monoclonal antibody M01172 available?
A. Thomas
Verified customer
Asked: 2014-01-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GCN2 Monoclonal antibody M01172 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-01-24
