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Product Info Summary
| SKU: | P00135 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00135
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody catalog # P00135. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00135)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DBD-5
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-eIF4E (S209)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00135 is reactive to EIF4E in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
70, 75 kDa
Calculated molecular weight
25097 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00135 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of eIF4E (phosphoS209) expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using Phospho-eIF4E (S209) antibody.
Protein Target Info & Infographic
Gene/Protein Information For EIF4E (Source: Uniprot.org, NCBI)
Gene Name
EIF4E
Full Name
Eukaryotic translation initiation factor 4E
Weight
25097 MW
Superfamily
eukaryotic initiation factor 4E family
Alternative Names
Eukaryotic translation initiation factor 4E;eIF-4E;eIF4E;eIF-4F 25 kDa subunit;mRNA cap-binding protein;EIF4E;EIF4EL1, EIF4F; EIF4E AUTS19, CBP1, EIF4EL1, EIF4F, eIF-4E, EIF4E eukaryotic translation initiation factor 4E eukaryotic translation initiation factor 4E|eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4E, check out the EIF4E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody (P00135)
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No publications found for P00135
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody
Question
Would P00135 react with Arabidopsis samples?
Verified customer
Asked: 2019-09-14
Answer
We haven't tested the Anti-Phospho-EIF4E (S209) Rabbit Monoclonal Antibody (P00135) on Arabidopsis samples. Please run a pilot test. Immunogen sequence for this antibody is CDTATKSG-pS-TTKNRFVV.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-Phospho-eIF4E (S209) Rabbit Monoclonal antibody P00135 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The anti-Phospho-eIF4E (S209) Rabbit Monoclonal antibody (P00135) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-15
