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Product Info Summary
| SKU: | A00968 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-4E-BP1(Ab-45) EIF4EBP1 Antibody
SKU/Catalog Number
A00968
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-4E-BP1(Ab-45) EIF4EBP1 Antibody (Catalog # A00968). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-4E-BP1(Ab-45) EIF4EBP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00968)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.44~48 (S-T-T-P-G) derived from Human 4E-BP1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00968 is reactive to EIF4EBP1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
12580 MW
Background of 4EBP1
4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Gingras AC, et al. (1998) Genes Dev 12(4): 502-513.
Brugarolas J, et al. (2004) Genes Dev 18(23): 2893-2904.
Kumar V, et al. (2000) EMBO J 19(5): 1087-1097.
Moody CA, et al. (2005) J Virol 79(9): 5499-5506.
Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95(4): 1432-1437.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00968 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 18kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1(Ab-45) antibody A00968(left) or the same antibody preincubated with blocking peptide(right).
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Western blot analysis of extracts from 293 and HepG2 cells using 4E-BP1(Ab-45) antibody A00968.
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Immunofluorescence staining of methanol-fixed Hela cells using 4E-BP1(Ab-45) antibody A00968.
Protein Target Info & Infographic
Gene/Protein Information For EIF4EBP1 (Source: Uniprot.org, NCBI)
Gene Name
EIF4EBP1
Full Name
Eukaryotic translation initiation factor 4E-binding protein 1
Weight
12580 MW
Superfamily
eIF4E-binding protein family
Alternative Names
Eukaryotic translation initiation factor 4E-binding protein 1;4E-BP1;eIF4E-binding protein 1;Phosphorylated heat- and acid-stable protein regulated by insulin 1;PHAS-I;EIF4EBP1; EIF4EBP1 4E-BP1, 4EBP1, BP-1, PHAS-I eukaryotic translation initiation factor 4E binding protein 1 eukaryotic translation initiation factor 4E-binding protein 1|eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EIF4EBP1, check out the EIF4EBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EIF4EBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-4E-BP1(Ab-45) EIF4EBP1 Antibody (A00968)
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No publications found for A00968
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