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Product Info Summary
| SKU: | M12995 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC |
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Product info
Product Name
Anti-GCET2 Monoclonal Antibody
SKU/Catalog Number
M12995
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GCET2 Monoclonal Antibody catalog # M12995. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCET2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12995)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFHF-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12995 is reactive to GCSAM in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
58 kDa
Calculated molecular weight
15404 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12995 is guaranteed for Flow Cytometry, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:100
Positive Control
IHC: human bladder cancer tissue
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GCET2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For GCSAM (Source: Uniprot.org, NCBI)
Gene Name
GCSAM
Full Name
Germinal center-associated signaling and motility protein
Weight
15404 MW
Alternative Names
Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCSAM, check out the GCSAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCSAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCET2 Monoclonal Antibody (M12995)
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No publications found for M12995
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Customer Q&As
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5 Customer Q&As for Anti-GCET2 Monoclonal Antibody
Question
We are currently using anti-GCET2 Monoclonal antibody M12995 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The anti-GCET2 Monoclonal antibody (M12995) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-03
Question
Does anti-GCET2 Monoclonal antibody M12995 work for IHC with lymphoma?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
According to the expression profile of lymphoma, GCSAM is highly expressed in lymphoma. So, it is likely that anti-GCET2 Monoclonal antibody M12995 will work for IHC with lymphoma.
Boster Scientific Support
Answered: 2017-10-09
Question
I am interested in to test anti-GCET2 Monoclonal antibody M12995 on human lymphoma for research purposes, then I may be interested in using anti-GCET2 Monoclonal antibody M12995 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Collins
Verified customer
Asked: 2017-01-12
Answer
The products we sell, including anti-GCET2 Monoclonal antibody M12995, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-01-12
Question
Is this M12995 anti-GCET2 Monoclonal antibody reactive to the isotypes of GCSAM?
P. Edwards
Verified customer
Asked: 2014-12-30
Answer
The immunogen of M12995 anti-GCET2 Monoclonal antibody is A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-12-30
Question
Will M12995 anti-GCET2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Patel
Verified customer
Asked: 2013-06-19
Answer
As indicated on the product datasheet, M12995 anti-GCET2 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-19
