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Product Info Summary
| SKU: | A05357-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GBL MLST8 Antibody
SKU/Catalog Number
A05357-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GBL MLST8 Antibody (Catalog # A05357-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GBL antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GBL MLST8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05357-1)
Host
Rabbit
Contents
GBL Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GbL antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human GbL. The immunogen is located within the first 50 amino acids of GBL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of GLS2 are known to exist.
Reactive Species
A05357-1 is reactive to Mlst8 in Human, Mouse, Rat
Applications
A05357-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
35876 MW
Background of GBL
GbetaL (G protein beta protein subunit-like) is a member of a signaling pathway that regulates mammalian cell growth in response to the presence of nutrients and growth factors. It binds to the kinase domain of TOR (Target of rapamycin, also known as mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with GbetaL and an additional regulatory protein RAPTOR, allowing TOR to control protein biosynthesis. The binding of GbetaL to TOR stimulates TOR's kinase activity towards downstream proteins such as RPS6K (ribosomal protein S6 kinase) and the translation factor 4E-BP1 which leads to increased protein translation and cell growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GbL antibody can be used for the detection of GbL by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GbL in human brain cell lysate with GbL antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of GbL in mouse brain tissue with GbL antibody at 10 μg/mL.
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Immunofluorescence of GBL in Mouse Brain cells with GBL antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Mlst8 (Source: Uniprot.org, NCBI)
Gene Name
Mlst8
Full Name
Target of rapamycin complex subunit LST8
Weight
35876 MW
Superfamily
WD repeat LST8 family
Alternative Names
G protein beta subunit-like; gable; GbetaL; GBL; GBLPop3; Lst8; LST8MGC111011; Mammalian lethal with SEC13 protein 8; mLST8; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1 Mlst8|0610033N12Rik, AA409454, AI505104, AI851821, Gb, Gbl, mLS|MTOR associated protein, LST8 homolog (S. cerevisiae)|target of rapamycin complex subunit LST8|TORC subunit LST8|mammalian lethal with SEC13 protein 8|protein GbetaL|transducin (beta)-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mlst8, check out the Mlst8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mlst8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GBL MLST8 Antibody (A05357-1)
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