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Product Info Summary
| SKU: | A05264 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-UBE2A/B Antibody
View all HR6A/UBE2A Antibodies
SKU/Catalog Number
A05264
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UBE2A/B Antibody catalog # A05264. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE2A/B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05264)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the C-terminal region of human UBE2A/B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05264 is reactive to UBE2A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
17315 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05264 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-300
Validation Images & Assay Conditions
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Western Blot (WB) analysis of extracts from Jurkat cells, using UBE2A/B Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For UBE2A (Source: Uniprot.org, NCBI)
Gene Name
UBE2A
Full Name
Ubiquitin-conjugating enzyme E2 A
Weight
17315 MW
Superfamily
ubiquitin-conjugating enzyme family
Alternative Names
Ubiquitin-conjugating enzyme E2 A;2.3.2.23 ;E2 ubiquitin-conjugating enzyme A;RAD6 homolog A;HR6A;hHR6A;Ubiquitin carrier protein A;Ubiquitin-protein ligase A;UBE2A;RAD6A; UBE2A HHR6A, MRXS30, MRXSN, RAD6A, UBC2 ubiquitin conjugating enzyme E2 A ubiquitin-conjugating enzyme E2 A|E2 ubiquitin-conjugating enzyme A|RAD6 homolog A|ubiquitin carrier protein A|ubiquitin-conjugating enzyme E2A|ubiquitin-protein ligase A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on UBE2A, check out the UBE2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-UBE2A/B Antibody (A05264)
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No publications found for A05264
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-UBE2A/B Antibody
Question
I would like to test anti-UBE2A/B antibody A05264 on mouse chorionic villus for research purposes, then I may be interested in using anti-UBE2A/B antibody A05264 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The products we sell, including anti-UBE2A/B antibody A05264, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-UBE2A/B antibody A05264 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
The anti-UBE2A/B antibody (A05264) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-29
Question
I was wanting to use your anti-UBE2A/B antibody for IHC for mouse chorionic villus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse chorionic villus identification?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
You can see on the product datasheet, A05264 anti-UBE2A/B antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse chorionic villus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-02
Question
Please see the WB image, lot number and protocol we used for chorionic villus using anti-UBE2A/B antibody A05264. Please let me know if you require anything else.
J. Patel
Verified customer
Asked: 2014-12-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-19
Question
Will anti-UBE2A/B antibody A05264 work for IHC with chorionic villus?
V. Krishna
Verified customer
Asked: 2014-07-01
Answer
According to the expression profile of chorionic villus, UBE2A is highly expressed in chorionic villus. So, it is likely that anti-UBE2A/B antibody A05264 will work for IHC with chorionic villus.
Boster Scientific Support
Answered: 2014-07-01
Question
Is this A05264 anti-UBE2A/B antibody reactive to the isotypes of UBE2A?
G. Jha
Verified customer
Asked: 2013-03-25
Answer
The immunogen of A05264 anti-UBE2A/B antibody is Synthesized peptide derived from the C-terminal region of human UBE2A/B. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-03-25
