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Key=meta_description, value=Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human. Supplied as 100ug in Liquid form antibody.
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Key=image_label, value=Western blot analysis of GATA4 expression in CCRF-CEM cell lysates (lane 1). GATA4 at 44.5KD was detected using mouse anti-GATA4 protein A purified monoclonal antibody (Catalog # M00499) at 1:500. The blot was developed using chemiluminescence (ECL) metho
Key=small_image_label, value=Western blot analysis of GATA4 expression in CCRF-CEM cell lysates (lane 1). GATA4 at 44.5KD was detected using mouse anti-GATA4 protein A purified monoclonal antibody (Catalog # M00499) at 1:500. The blot was developed using chemiluminescence (ECL) metho
Key=thumbnail_label, value=Western blot analysis of GATA4 expression in CCRF-CEM cell lysates (lane 1). GATA4 at 44.5KD was detected using mouse anti-GATA4 protein A purified monoclonal antibody (Catalog # M00499) at 1:500. The blot was developed using chemiluminescence (ECL) metho
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Key=size, value=100ug
Key=protein_name, value=Erythropoietin
Key=gene_full_name, value=Transcription factor GATA-4
Key=uniprot_id, value=P43694
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Key=reactivity, value=Human
Key=form, value=Liquid (sterile filtered)
Key=concentration, value=1.0mg/mL by UV absorbance at 280 nm
Key=clone_number, value=Clone: 10D8.B10.D4
Key=source_company, value=Rockland 200-301-P15; Cost: 358.4
Key=isotype, value=IgG1 kappa
Key=molecular_weight, value=44565 MW
Key=sample_size_available, value=25ug for $99, contact us for details
Key=related_product_skus, value=A00499-1,M00499-1
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Key=research_category, value=Cancer, Cardiovascular, Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Hypertrophy, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors, Tumor Suppressors, Zinc Finger
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Key=description, value=Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human.
Key=short_description, value=Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human.
Key=meta_keyword, value=Anti-GATA4 Monoclonal Antibody
Key=contents, value=0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Key=background, value=GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Key=gene_name, value=GATA4
Key=synonyms, value=GATA4, Transcription factor GATA-4, GATA-binding factor 4, GATA-4
Key=application_details, value=ELISA: 1:2000 - 1:10,000
WB: 1:500 - 1:1000
Key=subcellular_localization, value= Nucleus.
Key=tissue_specificity, value=Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
Key=protein_function, value= Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)- mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Key=application_notes, value=Anti-GATA4 antibody has been tested by ELISA, SDS-Page, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.
Key=competitor_equivalent_skus, value=sc 85135
Key=immunogen, value=GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Key=purification, value=Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.   A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.
Key=storage, value=Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
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Anti-GATA4 Monoclonal Antibody

GATA-4 antibody

Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: M00499
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: ELISA, WB

Product Name

Anti-GATA4 Monoclonal Antibody

View all GATA-4 Antibodies

SKU/Catalog Number

M00499

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-GATA4 Monoclonal Antibody (Catalog # M00499). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-GATA4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00499)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 10D8.B10.D4

Isotype

IgG1 kappa

Immunogen

GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00499 is reactive to GATA4 in Human

Reconstitution

Calculated molecular weight

44565 MW

Background of GATA-4

GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00499 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2000 - 1:10,000
WB: 1:500 - 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For GATA4 (Source: Uniprot.org, NCBI)

Gene Name

GATA4

Full Name

Transcription factor GATA-4

Weight

44565 MW

Alternative Names

GATA4, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 GATA4 ASD2, TACHD, TOF, VSD1 GATA binding protein 4 transcription factor GATA-4|GATA-binding factor 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GATA4, check out the GATA4 Infographic

GATA4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GATA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00499 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Differentiation of human embryonic germ cells and transplantation in rats with acute myocardial infarction

Liu J, Wang Y, Du W, Liu W, Liu F, Zhang L, Zhang M, Hou M, Liu K, Zhang S, Yu B. Plos One. 2013;8(3):E58883. Doi: 10.1371/Journal.Pone.0058883. Epub 2013 Mar 22. Wnt1 Inhibits Hydrogen Peroxide-Induced Apoptosis In Mouse Cardiac Stem Cells.

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5 Customer Q&As for Anti-GATA4 Monoclonal Antibody

Question

My question regarding product M00499, anti-GATA4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00499 anti-GATA4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-10

Question

Would M00499 anti-GATA4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

You can see on the product datasheet, M00499 anti-GATA4 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-17

Question

Would anti-GATA4 Monoclonal antibody M00499 work on dog WB with heart lung?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

Our lab technicians have not validated anti-GATA4 Monoclonal antibody M00499 on dog. You can run a BLAST between dog and the immunogen sequence of anti-GATA4 Monoclonal antibody M00499 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog heart lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-29

Question

Would anti-GATA4 Monoclonal antibody M00499 work for WB with heart lung?

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

According to the expression profile of heart lung, GATA4 is highly expressed in heart lung. So, it is likely that anti-GATA4 Monoclonal antibody M00499 will work for WB with heart lung.

Boster Scientific Support

Answered: 2019-04-18

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart lung using anti-GATA4 Monoclonal antibody M00499. Let me know if you need anything else.

F. Li

Verified customer

Asked: 2017-05-05

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-05

Order DetailsPrice
M00499-100uL

100uL

$646

Free Secondary Antibody

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M00499
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$646.00

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