Key=store_id Key=entity_id, value=79120 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00499-1 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2020-03-11 06:18:58 Key=updated_at, value=2024-09-26 01:05:29 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-GATA4 Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=Yes Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1364 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Anti-GATA4 Antibody Key=image, value=/a/0/a00499-1-1-western-blotting.jpg Key=small_image, value=/a/0/a00499-1-1-western-blotting.jpg Key=thumbnail, value=/a/0/a00499-1-1-western-blotting.jpg Key=gallery, value=/A00237_1.jpg:/A00237_2.jpg:/A00237_3.jpg Key=url_key, value=anti-gata4-antibody-a00499-1-boster Key=url_path, value=anti-cytochrome-p450-2d6-picoband-trade-antibody-a00498-1-boster Key=options_container, value=Block after Info Column Key=image_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp Key=small_image_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp Key=thumbnail_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp Key=msrp_display_actual_price_type, value=Use config Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=protein_name, value=Transcription factor GATA-4 Key=gene_full_name, value=Transcription factor GATA-4 Key=uniprot_id, value=P43694 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=applications, value=WB Key=reactivity, value=Human Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster A00499-1 Key=isotype, value=Rabbit IgG Key=molecular_weight, value=44565 MW Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00499-1-1-western-blotting.jpg Key=rating_value, value=100 Key=review_count, value=6 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_facebook_image, value=/a/0/a00499-1-1-western-blotting.jpg Key=yoast_twitter_image, value=/a/0/a00499-1-1-western-blotting.jpg Key=cj_related_products, value=Atrial Septal Defects:M00499|A00499S105|DZ10161^Mesenchymal Stem Cell Markers:FC00555-iFluor647^Tetralogy Of Fallot:PROTQ9Y5X2|A00778 Key=description, value=Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-GATA4 Antibody Key=contents, value=Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. Key=background, value=Transcription factor GATA-4 is a protein that in humans is encoded by the GATA4 gene. This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Key=gene_name, value=GATA4 Key=synonyms, value=Transcription factor GATA-4;GATA-binding factor 4;GATA4;
Key=application_details, value=Western blot, 0.1-0.5 μg/ml, Human
Key=subcellular_localization, value=Nucleus Key=tissue_specificity, value=Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells. Key=protein_function, value=Transcriptional activator that binds to the consensus
sequence 5'-AGATAG-3' and plays a key role in cardiac development
and function (PubMed:24000169, PubMed:27984724). In cooperation
with TBX5, it binds to cardiac super-enhancers and promotes
cardiomyocyte gene expression, while it downregulates endocardial
and endothelial gene expression (PubMed:27984724). Involved in
bone morphogenetic protein (BMP)-mediated induction of cardiac-
specific gene expression. Binds to BMP response element (BMPRE)
DNA sequences within cardiac activating regions (By similarity).
Acts as a transcriptional activator of ANF in cooperation with
NKX2-5 (By similarity). Promotes cardiac myocyte enlargement
(PubMed:20081228). Required during testicular development
(PubMed:21220346). May play a role in sphingolipid signaling by
regulating the expression of sphingosine-1-phosphate degrading
enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Key=application_notes, value=Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 30043|sc 390193|sc 23690|sc 130366 Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human GATA4, different from the related mouse and rat sequences by one amino acid. Key=purification, value=Immunogen affinity purified. Key=storage, value=At -20˚C for one year from date of receipt.
After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months.
Avoid repeated freezing and thawing. Key=custom_attribute_1, value=45 kDa Key=custom_attribute_2, value=WB: human Hela whole Cell Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-GATA4 Antibody Picoband®
GATA-4 antibody
Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).
Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20˚C for one year from date of receipt.
After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months.
Avoid repeated freezing and thawing.
Cite This Product
Anti-GATA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00499-1)
A synthetic peptide corresponding to a sequence at the C-terminus of human GATA4, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00499-1 is reactive to GATA4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
44565 MW
Background of GATA-4
Transcription factor GATA-4 is a protein that in humans is encoded by the GATA4 gene. This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5 μg/ml, Human
Positive Control
WB: human Hela whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on GATA4, check out the GATA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GATA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.