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Key=image_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
Key=small_image_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
Key=thumbnail_label, value=Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of samp
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Key=size, value=100 μg/vial
Key=protein_name, value=Transcription factor GATA-4
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Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Key=predicted_reactivity
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Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=44565 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Pathways and Processes, Signal Transduction
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Atrial Septal Defects:M00499|A00499S105|DZ10161^Mesenchymal Stem Cell Markers:FC00555-iFluor647^Tetralogy Of Fallot:PROTQ9Y5X2|A00778
Key=description, value=Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-GATA4 Antibody
Key=contents, value=Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
Key=background, value=Transcription factor GATA-4 is a protein that in humans is encoded by the GATA4 gene. This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types.
Key=gene_name, value=GATA4
Key=synonyms, value=Transcription factor GATA-4;GATA-binding factor 4;GATA4;
Key=application_details, value=Western blot, 0.1-0.5 μg/ml, Human

Key=subcellular_localization, value=Nucleus
Key=tissue_specificity, value=Widely expressed in hematopoietic cells. Expressed in neutrophils. Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells.
Key=protein_function, value=Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac- specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 30043|sc 390193|sc 23690|sc 130366
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human GATA4, different from the related mouse and rat sequences by one amino acid.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=At -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freezing and thawing.
Key=custom_attribute_1, value=45 kDa
Key=custom_attribute_2, value=WB: human Hela whole Cell
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Anti-GATA4 Antibody Picoband®

GATA-4 antibody

Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A00499-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-GATA4 Antibody Picoband®

View all GATA-4 Antibodies

SKU/Catalog Number

A00499-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GATA4 Antibody catalog # A00499-1. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GATA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00499-1)

Host

Rabbit

Contents

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GATA4, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00499-1 is reactive to GATA4 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

45 kDa

Calculated molecular weight

44565 MW

Background of GATA-4

Transcription factor GATA-4 is a protein that in humans is encoded by the GATA4 gene. This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00499-1 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5 μg/ml, Human

Positive Control

WB: human Hela whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For GATA4 (Source: Uniprot.org, NCBI)

Gene Name

GATA4

Full Name

Transcription factor GATA-4

Weight

44565 MW

Alternative Names

Transcription factor GATA-4;GATA-binding factor 4;GATA4; GATA4 ASD2, TACHD, TOF, VSD1 GATA binding protein 4 transcription factor GATA-4|GATA-binding factor 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GATA4, check out the GATA4 Infographic

GATA4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GATA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00499-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Recombinant human brain natriuretic peptide therapy combined with bone mesenchymal stem cell transplantation for treating heart failure in rats

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Order DetailsPrice
A00499-1

100μg

$370
A00499-1-10ug

10μg sample (liquid)

$99
A00499-1-Biotin

100 μg Biotin conjugated

$570
A00499-1-Cy3

100 μg Cy3 conjugated

$570
A00499-1-Dylight488

100 μg Dylight488 conjugated

$570
A00499-1-Dylight550

100 μg Dylight550 conjugated

$570
A00499-1-Dylight594

100 μg Dylight594 conjugated

$570
A00499-1-FITC

100 μg FITC conjugated

$570
A00499-1-HRP

100 μg HRP conjugated

$570
A00499-1-APC

100 μg APC conjugated

$670
A00499-1-PE

100 μg PE conjugated

$670
A00499-1-iFluor647

100 μg iFluor647 conjugated

$670
A00499-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A00499-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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