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Product Info Summary
| SKU: | PB9096-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF |
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Product info
Product Name
Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody
SKU/Catalog Number
PB9096-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody catalog # PB9096-Dyl488. Tested in Flow Cytometry, IF applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9096-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD45 recombinant protein (Position: R1113-S1304). Human CD45 shares 68% amino acid (aa) sequence identity with both mouse and rat CD45.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9096-Dyl488 is reactive to PTPRC in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
147254 MW
Background of PTPRC
CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9096-Dyl488 is guaranteed for Flow Cytometry, IF Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
IF: human tonsil tissue, human tonsil tissue
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of THP-1 cells using anti-CD45 antibody (PB9096-Dyl488).
Overlay histogram showing THP-1 cells stained with PB9096-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD45 Antibody (PB9096-Dyl488,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 2. IF analysis of CD45 using anti-CD45 antibody (PB9096-Dyl488)
CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD45 Antibody (PB9096-Dyl488) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. IF analysis of CD45 using anti-CD45 antibody (PB9096-Dyl488)
CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD45 Antibody (PB9096-Dyl488) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)
Gene Name
PTPRC
Full Name
Receptor-type tyrosine-protein phosphatase C
Weight
147254 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45; PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 |T200 glycoprotein|T200 leukocyte common |protein tyrosine phosphatase, receptor type, c polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPRC, check out the PTPRC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody (PB9096-Dyl488)
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Customer Q&As
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4 Customer Q&As for Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody
Question
We have seen staining in human erythroleukemia. What should we do? Is anti-Human CD45 DyLight® 488 conjugated antibody supposed to stain erythroleukemia positively?
L. Mangal
Verified customer
Asked: 2019-10-25
Answer
From what I have seen in literature erythroleukemia does express PTPRC. From what I have seen in Uniprot.org, PTPRC is expressed in blood, lymphocyte, synovium, placenta, t-cell, liver, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-10-25
Question
Our team were well pleased with the WB result of your anti-Human CD45 DyLight® 488 conjugated antibody. However we have observed positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is PTPRC supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
According to literature, liver does express PTPRC. Generally PTPRC expresses in cell membrane. Regarding which tissues have PTPRC expression, here are a few articles citing expression in various tissues:
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218
Lymphocyte, Pubmed ID: 2824653
Placenta, Pubmed ID: 2971730, 2845400
Synovium, Pubmed ID: 14702039
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-05-10
Question
We are currently using anti-Human CD45 DyLight® 488 conjugated antibody PB9096-Dyl488 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
The anti-Human CD45 DyLight® 488 conjugated antibody (PB9096-Dyl488) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-06-09
Question
Our lab used your anti-Human CD45 DyLight® 488 conjugated antibody for Flow Cytometry on t-cell a few months ago. I am using human, and We want to use the antibody for IF next. We are interested in examining t-cell as well as leukemic t-cell in our next experiment. Could give a recommendation on which antibody would work the best for IF?
R. Mitchell
Verified customer
Asked: 2014-01-02
Answer
I have checked the website and datasheets of our anti-Human CD45 DyLight® 488 conjugated antibody and it appears that PB9096-Dyl488 has been tested on human in both Flow Cytometry and IF. Thus PB9096-Dyl488 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-01-02
