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Product Info Summary
| SKU: | A12630-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-GAREM1 Antibody Picoband®
SKU/Catalog Number
A12630-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAREM1 Antibody Picoband® catalog # A12630-1. Tested in WB, IHC, FCM, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GAREM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12630-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GAREM1 recombinant protein (Position: Q277-E753). Human GAREM1 shares 86.8% amino acid (aa) sequence identity with mouse GAREM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12630-1 is reactive to GAREM1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
97 kDa
Calculated molecular weight
97.186kDa
Background of FAM59A
This gene encodes an adaptor protein which functions in the epidermal growth factor (EGF) receptor-mediated signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12630-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry, 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human SH-SY5Y whole cell, human RT4 whole cell, human Hacat whole cell, human SIHA whole cell
IHC: human liver cancer tissue, human liver cancer tissue
FCM: SH-SY5Y cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GAREM1 using anti-GAREM1 antibody (A12630-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAREM1 antigen affinity purified polyclonal antibody (Catalog # A12630-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAREM1 at approximately 97 kDa. The expected band size for GAREM1 is at 97 kDa.
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Figure 2. IHC analysis of GAREM1 using anti-GAREM1 antibody (A12630-1).
GAREM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAREM1 Antibody (A12630-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of GAREM1 using anti-GAREM1 antibody (A12630-1).
GAREM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAREM1 Antibody (A12630-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of SH-SY5Y cells using anti-GAREM1 antibody (A12630-1).
Overlay histogram showing SH-SY5Y cells stained with A12630-11 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GAREM1 Antibody (A12630-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For GAREM1 (Source: Uniprot.org, NCBI)
Gene Name
GAREM1
Full Name
GRB2-associated and regulator of MAPK protein 1
Weight
97.186kDa
Superfamily
GAREM family
Alternative Names
Chromosome 18 Open Reading Frame 11; chromosome 18 open reading frame 11, family with sequence; FAM59A; Family With Sequence Similarity 59, Member A; FLJ21610; GAREM; Gm944; GRB2 associated, regulator of MAPK1; Grb2-Associated And Regulator Of Erk/MAPK; GRB2-Associated And Regulator Of MAPK Protein; GRB2-Associated And Regulator Of MAPK1; Protein FAM59A GAREM1 C18orf11, FAM59A, GAREM, Gm944 GRB2 associated regulator of MAPK1 subtype 1 GRB2-associated and regulator of MAPK protein 1|GRB2 associated regulator of MAPK1 1|GRB2 associated, regulator of MAPK1|GRB2-associated and regulator of MAPK1|Grb2-associated and regulator of Erk/MAPK|family with sequence similarity 59, member A|protein FAM59A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAREM1, check out the GAREM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAREM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAREM1 Antibody Picoband® (A12630-1)
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