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Product Info Summary
| SKU: | A02394-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-GADD34/PPP1R15A Antibody Picoband®
SKU/Catalog Number
A02394-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GADD34/PPP1R15A Antibody Picoband® catalog # A02394-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GADD34/PPP1R15A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02394-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GADD34/PPP1R15A recombinant protein (Position: A574-E602).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02394-2 is reactive to PPP1R15A in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
71.84kDa
Background of GADD34
Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02394-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human HEL whole cell, human RT4 whole cell, human U251 whole cell, human SIHA whole cell, human U-87MG whole cell, human PC-3 whole cell, rat testis tissue
IHC: human melanom tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: human SIHA whole cell lysates,
Lane 6: human U-87MG whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD34/PPP1R15A antigen affinity purified polyclonal antibody (Catalog # A02394-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD34/PPP1R15A at approximately 100 kDa. The expected band size for GADD34/PPP1R15A is at 73 kDa.
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Figure 2. IHC analysis of GADD34/PPP1R15A using anti-GADD34/PPP1R15A antibody (A02394-2).
GADD34/PPP1R15A was detected in a paraffin-embedded section of human melanom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GADD34/PPP1R15A Antibody (A02394-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R15A (Source: Uniprot.org, NCBI)
Gene Name
PPP1R15A
Full Name
Protein phosphatase 1 regulatory subunit 15A
Weight
71.84kDa
Superfamily
PPP1R15 family
Alternative Names
Caspase-2; CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2; NEDD-2; Protease ICH-1; Caspase-2 subunit p18; Caspase-2 subunit p13; Caspase-2 subunit p12; CASP2; ICH1; NEDD2 Ppp1r15a|9630030H21, Gadd3, Gadd34, Myd116|protein phosphatase 1, regulatory subunit 15A|protein phosphatase 1 regulatory subunit 15A|growth arrest and DNA damage-inducible protein GADD34|growth arrest and DNA-damage-inducible 34|myeloid differentiation primary response gene 116|myeloid differentiation primary response protein MyD116|protein phosphatase 1, regulatory (inhibitor) subunit 15A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R15A, check out the PPP1R15A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R15A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GADD34/PPP1R15A Antibody Picoband® (A02394-2)
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