Product Info Summary
SKU: | M02394 |
---|---|
Size: | 100 µl |
Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI3D12]
SKU/Catalog Number
M02394
Size
100 µl
Description
Boster Bio PPP1R15A (GADD34) mouse monoclonal antibody, clone OTI3D12 (formerly 3D12). Catalog# M02394. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI3D12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02394)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3D12
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human PPP1R15A (NP_055145) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02394 is reactive to PPP1R15A in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
71.84kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02394 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPP1R15A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either PPP1R15A (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPP1R15A antibody (M02394)
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Anti-PPP1R15A mouse monoclonal antibody (M02394) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R15A (Source: Uniprot.org, NCBI)
Gene Name
PPP1R15A
Full Name
Protein phosphatase 1 regulatory subunit 15A
Weight
71.84kDa
Superfamily
PPP1R15 family
Alternative Names
GADD34Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; Myeloid differentiation primary response protein MyD116 homolog; protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A Ppp1r15a|9630030H21, Gadd3, Gadd34, Myd116|protein phosphatase 1, regulatory subunit 15A|protein phosphatase 1 regulatory subunit 15A|growth arrest and DNA damage-inducible protein GADD34|growth arrest and DNA-damage-inducible 34|myeloid differentiation primary response gene 116|myeloid differentiation primary response protein MyD116|protein phosphatase 1, regulatory (inhibitor) subunit 15A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R15A, check out the PPP1R15A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R15A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GADD34 (PPP1R15A) Mouse Monoclonal Antibody [Clone ID: OTI3D12] (M02394)
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