Click image to see more details
Product Info Summary
| SKU: | M32410-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FYB Rabbit Monoclonal Antibody
View all FYB/ADAP/SLAP130 Antibodies
SKU/Catalog Number
M32410-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-FYB Rabbit Monoclonal Antibody catalog # M32410-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FYB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32410-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20F94
Isotype
IgG
Immunogen
A synthesized peptide derived from human FYB
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M32410-1 is reactive to FYB1 in Human, Mouse
Reconstitution
Observed Molecular Weight
120 kDa
Calculated molecular weight
26053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M32410-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human TF-1 whole cell, human Jurkat whole cell, human HEL whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FYB using anti-FYB antibody (M32410-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human TF-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FYB antigen affinity purified monoclonal antibody (Catalog # M32410-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FYB at approximately 120 kDa. The expected band size for FYB is at 85 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FYB1 (Source: Uniprot.org, NCBI)
Gene Name
FYB1
Full Name
FYN-binding protein 1
Weight
26053 MW
Alternative Names
Red fluorescent protein eqFP611;GFP-like chromoprotein; FYB1 ADAP, FYB, PRO0823, SLAP-130, SLAP130, THC3 FYN binding protein 1 FYN-binding protein 1|FYB-120/130|FYN-T-binding protein|SLP-76-associated phosphoprotein|adhesion and degranulation-promoting adaptor protein|p120/p130
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FYB1, check out the FYB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FYB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FYB Rabbit Monoclonal Antibody (M32410-1)
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