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Product Info Summary
| SKU: | M04370-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-Fructose 6 Phosphate Kinase Rabbit Monoclonal Antibody
View all Muscle Phosphofructokinase/PFKM/PFK-1 Antibodies
SKU/Catalog Number
M04370-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Fructose 6 Phosphate Kinase Rabbit Monoclonal Antibody catalog # M04370-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fructose 6 Phosphate Kinase Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04370-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
18P49
Isotype
IgG
Immunogen
A synthesized peptide derived from human Fructose 6 Phosphate Kinase
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04370-1 is reactive to PFKM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
81 kDa
Calculated molecular weight
48507 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04370-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:100
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, rat heart tissue, rat brain tissue, mouse heart tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PFKM using anti-PFKM antibody (M04370-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKM antigen affinity purified monoclonal antibody (M04370-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKM at approximately 81 kDa. The expected band size for PFKM is at 85 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PFKM (Source: Uniprot.org, NCBI)
Gene Name
PFKM
Full Name
ATP-dependent 6-phosphofructokinase, muscle type
Weight
48507 MW
Superfamily
phosphofructokinase type A (PFKA) family
Alternative Names
Urokinase-type plasminogen activator;U-plasminogen activator;uPA;3.4.21.73;Urokinase-type plasminogen activator long chain A;Urokinase-type plasminogen activator short chain A;Urokinase-type plasminogen activator chain B;PLAU; PFKM ATP-PFK, GSD7, PFK-1, PFK-A, PFK1, PFKA, PFKX, PPP1R122 phosphofructokinase, muscle ATP-dependent 6-phosphofructokinase, muscle type|6-phosphofructo-1-kinase|6-phosphofructokinase type A|6-phosphofructokinase, muscle type|phosphofructo-1-kinase isozyme A|phosphofructokinase 1|phosphofructokinase, polypeptide X|phosphofructokinase-M|phosphohexokinase|protein phosphatase 1, regulatory subunit 122
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKM, check out the PFKM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fructose 6 Phosphate Kinase Rabbit Monoclonal Antibody (M04370-1)
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