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Product Info Summary
| SKU: | A04370 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PFKM/Pfk 1 Antibody
View all Muscle Phosphofructokinase/PFKM/PFK-1 Antibodies
SKU/Catalog Number
A04370
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PFKM/Pfk 1 Antibody catalog # A04370. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKM/Pfk 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04370)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PFK-1. AA range:320-369
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04370 is reactive to PFKM in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
85183 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04370 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using PFKM Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PFKM (Source: Uniprot.org, NCBI)
Gene Name
PFKM
Full Name
ATP-dependent 6-phosphofructokinase, muscle type
Weight
85183 MW
Superfamily
phosphofructokinase type A (PFKA) family
Alternative Names
ATP-dependent 6-phosphofructokinase, muscle type ;ATP-PFK ;PFK-M;2.7.1.11 ;6-phosphofructokinase type A;Phosphofructo-1-kinase isozyme A;PFK-A;Phosphohexokinase ;PFKM;PFKX; PFKM ATP-PFK, GSD7, PFK-1, PFK-A, PFK1, PFKA, PFKX, PPP1R122 phosphofructokinase, muscle ATP-dependent 6-phosphofructokinase, muscle type|6-phosphofructo-1-kinase|6-phosphofructokinase type A|6-phosphofructokinase, muscle type|phosphofructo-1-kinase isozyme A|phosphofructokinase 1|phosphofructokinase, polypeptide X|phosphofructokinase-M|phosphohexokinase|protein phosphatase 1, regulatory subunit 122
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFKM, check out the PFKM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFKM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PFKM/Pfk 1 Antibody (A04370)
Hello CJ!
No publications found for A04370
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-PFKM/Pfk 1 Antibody
Question
My question regarding product A04370, anti-PFKM/Pfk 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04370 anti-PFKM/Pfk 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
Does anti-PFKM/Pfk 1 antibody A04370 work on bovine WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Our lab technicians have not validated anti-PFKM/Pfk 1 antibody A04370 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PFKM/Pfk 1 antibody A04370 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
I am looking for to test anti-PFKM/Pfk 1 antibody A04370 on rat thymus for research purposes, then I may be interested in using anti-PFKM/Pfk 1 antibody A04370 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The products we sell, including anti-PFKM/Pfk 1 antibody A04370, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-08
Question
Do you have a BSA free version of anti-PFKM/Pfk 1 antibody A04370 available?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PFKM/Pfk 1 antibody A04370 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-18
Question
Does anti-PFKM/Pfk 1 antibody A04370 work for WB with thymus?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of thymus, PFKM is highly expressed in thymus. So, it is likely that anti-PFKM/Pfk 1 antibody A04370 will work for WB with thymus.
Boster Scientific Support
Answered: 2019-07-25
Question
I see that the anti-PFKM/Pfk 1 antibody A04370 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-04
Question
Is a blocking peptide available for product anti-PFKM/Pfk 1 antibody (A04370)?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
We do provide the blocking peptide for product anti-PFKM/Pfk 1 antibody (A04370). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-10
Question
Here is the WB image, lot number and protocol we used for thymus using anti-PFKM/Pfk 1 antibody A04370. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-23
Question
Would A04370 anti-PFKM/Pfk 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
It shows on the product datasheet, A04370 anti-PFKM/Pfk 1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-19
Question
Is this A04370 anti-PFKM/Pfk 1 antibody reactive to the isotypes of PFKM?
J. Wu
Verified customer
Asked: 2017-06-13
Answer
The immunogen of A04370 anti-PFKM/Pfk 1 antibody is Synthesized peptide derived from the Internal region of human PFKM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-PFKM/Pfk 1 antibody A04370. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-24
Question
We are currently using anti-PFKM/Pfk 1 antibody A04370 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
C. Edwards
Verified customer
Asked: 2016-08-24
Answer
The anti-PFKM/Pfk 1 antibody (A04370) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-08-24
Question
I was wanting to use your anti-PFKM/Pfk 1 antibody for WB for rat thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat thymus identification?
L. Zhang
Verified customer
Asked: 2013-11-25
Answer
It shows on the product datasheet, A04370 anti-PFKM/Pfk 1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-25
