Anti-Phospho-Fragile X Mental Retardation Protein (FMRP) (Ser499) FMR1 Antibody

Product Name

Anti-Phospho-Fragile X Mental Retardation Protein (FMRP) (Ser499) FMR1 Antibody

View all FMRP Antibodies

SKU/Catalog Number

P00192

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-Fragile X Mental Retardation Protein (FMRP) (Ser499) FMR1 Antibody (Catalog # P00192). Tested in WB, IHC applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-Fragile X Mental Retardation Protein (FMRP) (Ser499) FMR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00192)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMRP, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00192 is reactive to FMR1 in Chicken, Human, Mouse, Rat

Applications

P00192 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

68989 MW

Background of FMRP

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser-499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:250-1:1000

Validation Images & Assay Conditions

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71kDa FMRP protein phosphorylated at Ser⁴⁹⁹ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Gene/Protein Information For FMR1 (Source: Uniprot.org, NCBI)

Alternative Names

FMRPFRAXAPOF; fragile X mental retardation 1 protein; fragile X mental retardation 1; MGC87458; POF1; premature ovarian failure 1; Protein FMR-1 FMR1 FMRP, FRAXA, POF, POF1 FMRP translational regulator 1 synaptic functional regulator FMR1|fragile X mental retardation 1|fragile X mental retardation protein 1|truncated FMRP

For more info on FMR1, check out the FMR1 Infographic

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In this infographic, you will see the following information for FMR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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