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Product Info Summary
| SKU: | A04942-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-FOXJ1 Antibody Picoband®
View all FoxJ1/HFH4 Antibodies
SKU/Catalog Number
A04942-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXJ1 Antibody Picoband® catalog # A04942-3. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FOXJ1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04942-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human FOXJ1 recombinant protein (Position: D27-A405).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04942-3 is reactive to FOXJ1 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
56903 MW
Background of FoxJ1/HFH4
Forkhead box protein J1 is a protein that in humans is encoded by the FOXJ1 gene. This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04942-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SW579 whole cell, human SK-OV-3 whole cell, rat PC-12 whole cell
IHC: rat ovary tissue
ICC/IF: CACO-2 cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXJ1 antigen affinity purified polyclonal antibody (Catalog # A04942-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXJ1 at approximately 55 kDa. The expected band size for FOXJ1 is at 55 kDa.
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Figure 2. IHC analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-3).
FOXJ1 was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FOXJ1 Antibody (A04942-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IF analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-3).
FOXJ1 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-FOXJ1 Antibody (A04942-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 4. Flow Cytometry analysis of HepG2 cells using anti-FOXJ1 antibody (A04942-3).
Overlay histogram showing HepG2 cells stained with A04942-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXJ1 Antibody (A04942-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For FOXJ1 (Source: Uniprot.org, NCBI)
Gene Name
FOXJ1
Full Name
Forkhead box protein J1
Weight
56903 MW
Superfamily
FOXJ1 family
Alternative Names
Meiotic recombination protein REC8 homolog; Cohesin Rec8p; REC8; REC8L1 FOXJ1 CILD43, FKHL13, HFH-4, HFH4 forkhead box J1 forkhead box protein J1|fork head homologue 4|forkhead transcription factor HFH-4|forkhead-like 13|forkhead-related protein FKHL13|hepatocyte nuclear factor 3 forkhead homolog 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXJ1, check out the FOXJ1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXJ1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXJ1 Antibody Picoband® (A04942-3)
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