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Product Info Summary
| SKU: | A01541-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-TLR8 Antibody
SKU/Catalog Number
A01541-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TLR8 Antibody catalog # A01541-3. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TLR8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01541-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at N-terminus of human TLR8, which shares 76.5% amino acid (aa) sequence identity with mouse TLR8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01541-3 is reactive to TLR8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
119.828kDa
Background of TLR8
TLR8 (Toll-like receptor 8) is a protein that in humans is encoded by the TLR8 gene. TLR8 has also been designated as CD288 (cluster of differentiation 288).The TLR8 gene is mapped to Xp22.3-p22.2 by Chuang and Ulevitch (2000) and Du et al. (2000). The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 recognises G-rich oligonucleotides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01541-3 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Positive Control
IHC: human gastric cancer tissue, human tonsil tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of TLR8 using anti-TLR8 antibody (A01541-3).
TLR8 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLR8 Antibody (A01541-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of TLR8 using anti-TLR8 antibody (A01541-3).
TLR8 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TLR8 Antibody (A01541-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TLR8 (Source: Uniprot.org, NCBI)
Gene Name
TLR8
Full Name
Toll-like receptor 8
Weight
119.828kDa
Superfamily
Toll-like receptor family
Alternative Names
Nuclear factor 1 B-type; NF1-B; Nuclear factor 1/B; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/B; NF-I/B; NFI-B; TGGCA-binding protein; NFIB TLR8 CD288 toll like receptor 8 toll-like receptor 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR8, check out the TLR8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TLR8 Antibody (A01541-3)
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