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- Table of Contents
Facts about Dimethylaniline monooxygenase [N-oxide-forming] 1.
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Human | |
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Gene Name: | FMO1 |
Uniprot: | Q01740 |
Entrez: | 2326 |
Belongs to: |
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FMO family |
dimethylaniline monooxygenase [N-oxide-forming] 1; Dimethylaniline oxidase 1; EC 1.14.13.8; Fetal hepatic flavin-containing monooxygenase 1; flavin containing monooxygenase 1; Flavin-containing monooxygenase 1 (fetal liver); FMO 1
Mass (kDA):
60.311 kDA
Human | |
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Location: | 1q24.3 |
Sequence: | 1; NC_000001.11 (171248471..171285978) |
Expressed mainly in fetal and adult liver.
Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
This article will discuss the phyllobacteria QPCR analysis and FMO1 variant detection. The optimization tips and guides in this article will help you optimize your experiment. Read on to learn more! You'll be glad to have done it. We'll also discuss ways to optimize your experiments in future. Until then, check out these tips.
Phyllosphere bacteria can induce rice to express several defense gene. These genes are expressed in high numbers at 48-hpi but drop sharply at 72hpi. OsCEBiP (OsNPR1), OsPDF2.2 (OsFMO1) and OsCEBiP were all expressed in the same way in all six phyllosphere-bacterial species.
The study found a functional core-phyllomicrobiome and identified the drivers of this assembly. Phyllosphere phyllomicrobiome profiles of rice genotypes from two different agro-climatic zones were compared. Proteobacteria prevailed in the mountain zone, while Firmicutes dominated. Core-microbiome analysis revealed associations with Acidovorax Arthrobacter, Bacillus, and Bacillus.
The phyllosphere's core microbiome has a close association with the plant that produces it. This microbiome is vertically transmitted by successive generations. Although there are many biotic, as well as abiotic, factors that influence the structure of this microbiome and its function in plant growth and developmental processes, it is still poorly understood. This study suggests that the microbiome of the phyllosphere is influenced both by macroclimatic and other abiotic factors. Moreover, the phyllosphere is a habitat for several prokaryotic phyla that have not been explored for functional characterization.
After comparing the two strands of DNA, the SPS gene was selected as the endogenous reference. This gene only had one copy in transgenic rice. This allowed for the detection of the rice SPS gene in practical samples at high sensitivity. Further research is needed to confirm these results and create a more robust quantitativePCR method for transgenic Rice detection.
To determine if bacterial populations interact with the host plant, the differential gene expression of bacterial strains from the late-exponential period was used. This study revealed that acdS encodes protein that inhibits root growth, and increases systemic resistance against pathogens. Bacterial degradation of acdS could result in a decrease in ethylene levels in the roots of plants and increased root development.
The results showed that genes were differentially expressed across the four bacterial strains. The gene expression levels of the genes were significantly different for strains B510 and 4B. A few genes that regulate the response to biobiotic stress were also differentially expressing in different combinations. These genes are involved in protein fate, transport, primary metabolism, transcription regulation, and transport. The genes involved in PR-gene transcription in rice were different in strain 4B than in strain B510.
There were also differences in the relative expression values for the genes that regulate the production and activity of these enzymes among the bacterial forms. The results were also validated by evaluating the expression of the antioxidant gene. This study used soil samples from a BRS Pampa strain. The qPCR data demonstrated that CATC is controlled by different genes.
Boster Bio has optimized the qPCR assays to use the FMO1 marker in order to detect Rice Sheath Blight. This necrotrophic fungal pathogen has been identified as the causal agent of rice sheath disease. Pretreatment with salicylic acid can stop it from growing. It can also be detected with picogram-level sensitivities. It is accessible through tebubio.
Recent research reported the detection of two FMO1 variants within rat brain and kidney tissue. Two antibodies against FMO1 was generated and affinity purified using the rat's cDNA. These antibodies recognized FMO4 proteins from rats and allowed for reactivity against their correct molecular mass. Furthermore, they showed no cross-reactivity against alternate human cDNA expressed FMO1 isoforms.
PCR can detect FMO1 variants of FMO1 in tissue samples. The Boster Bio FMO1 marker provides a specific gene-specific probe for a variety of tissues. This marker is designed to detect variants resulting from alternative splicing the FMO1 gene. In some cases, the variant might be indicative of a functional difference and warrants further research.
Boster Bio's FMO1 gene has a long list if targets. It predicted the targets from 102 genes, which includes hsa–miR-181a-3p. The targets of these miRNAs include CDh23, IPO8, AASS, H3F3B, COL16A1, ERCC5, HAXA9, COL16A1, and PDIA6.
This marker is used to detect Rhizoctonia Solani, a fungal pathogen that causes rice sheath blight. Pretreatment with salicylic acids prevents Rhizoctonia solani from growing. The FMO1 genetic link is also to the production CYC/TB1 & CIN/TCPs.
PMID: 1712018 by Dolphin C.T., et al. Cloning, primary sequence, and chromosomal mapping of a human flavin- containing monooxygenase (FMO1).
PMID: 12527699 by Furnes B., et al. Identification of novel variants of the flavin-containing monooxygenase gene family in African Americans.