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Product Info Summary
| SKU: | A04987-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Flt3 ligand/Flt3lg Antibody Picoband®
View all Flt-3 Ligand/FLT3L Antibodies
SKU/Catalog Number
A04987-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Flt3 ligand/Flt3lg Antibody Picoband® catalog # A04987-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flt3 ligand/Flt3lg Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04987-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Flt3 ligand recombinant protein (Position: T28-Q189).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04987-1 is reactive to Flt3lg in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
25847 MW
Background of Flt-3 Ligand/FLT3L
FLT3LG (FMS-Related Tyrosine Kinase 3 Ligand) also called FLT3 LIGAND, FL or FLT3L, is a protein which in humans is encoded by the FLT3LG gene. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt3 ligand (FL) is a hematopoietic four helical bundlecytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Lyman et al. (1993) found that Flt3 ligand stimulated proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Hannum et al. (1994) concluded that FL enhances the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. Assays of C-terminally truncated FL proteins confirmed that the N-terminal half conferred FL activity. Depletion of the Pi3k -Mtor negative regulator Pten facilitated Flt3l-driven DC development in culture.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04987-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse spleen tissue, mouse kidney tissue, rat skeletal muscle tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Flt3 ligand using anti-Flt3 ligand antibody (A04987-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse kidney tissue lysates,
Lane 3: rat skeletal muscle tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 ligand antigen affinity purified polyclonal antibody (Catalog # A04987-1) at 0.5 ug/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 ligand at approximately 26KD. The expected band size for Flt3 ligand is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For Flt3lg (Source: Uniprot.org, NCBI)
Gene Name
Flt3lg
Full Name
Fms-related tyrosine kinase 3 ligand
Weight
25847 MW
Alternative Names
Fms-related tyrosine kinase 3 ligand ;Flt3lg ; FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Flt3lg, check out the Flt3lg Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Flt3lg: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flt3 ligand/Flt3lg Antibody Picoband® (A04987-1)
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