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Product Info Summary
| SKU: | PA1914 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Flt3 ligand/FLT3LG Antibody Picoband®
View all Flt-3 Ligand/FLT3L Antibodies
SKU/Catalog Number
PA1914
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Flt3 ligand/FLT3LG Antibody catalog # PA1914. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flt3 ligand/FLT3LG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1914)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Flt-3ligand.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1914 is reactive to FLT3LG in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
26416 MW
Background of Flt-3 Ligand/FLT3L
FLT3LG (FMS-Related Tyrosine Kinase 3 Ligand) also called FLT3 LIGAND, FL or FLT3L, is a protein which in humans is encoded by the FLT3LG gene. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt3 ligand (FL) is a hematopoietic four helical bundlecytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Lyman et al. (1993) found that Flt3 ligand stimulated proliferation of hematopoietic progenitor cells isolated from mouse fetal liver or adult mouse bone marrow. Hannum et al. (1994) concluded that FL enhances the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells. Assays of C-terminally truncated FL proteins confirmed that the N-terminal half conferred FL activity. Depletion of the Pi3k -Mtor negative regulator Pten facilitated Flt3l-driven DC development in culture.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1914 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human PC-3 whole cell, human Caco-2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FLT3LG using anti-FLT3LG antibody (PA1914).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT3LG antigen affinity purified polyclonal antibody (Catalog # PA1914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT3LG at approximately 30KD. The expected band size for FLT3LG is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For FLT3LG (Source: Uniprot.org, NCBI)
Gene Name
FLT3LG
Full Name
Fms-related tyrosine kinase 3 ligand
Weight
26416 MW
Alternative Names
Fms-related tyrosine kinase 3 ligand;Flt3 ligand;Flt3L;SL cytokine;FLT3LG; FLT3LG FL, FLG3L, FLT3L fms related receptor tyrosine kinase 3 ligand fms-related tyrosine kinase 3 ligand|flt3 ligand|fms related tyrosine kinase 3 ligand
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLT3LG, check out the FLT3LG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLT3LG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flt3 ligand/FLT3LG Antibody Picoband® (PA1914)
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No publications found for PA1914
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Customer Q&As
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7 Customer Q&As for Anti-Flt3 ligand/FLT3LG Antibody Picoband®
Question
Is this PA1914 anti-Flt3 ligand/FLT3LG antibody reactive to the isotypes of FLT3LG?
M. Thomas
Verified customer
Asked: 2020-05-08
Answer
The immunogen of PA1914 anti-Flt3 ligand/FLT3LG antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Flt-3ligand(206-220aa HWQRTRRRTPRPGEQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-08
Question
See below the WB image, lot number and protocol we used for leukocyte using anti-Flt3 ligand/FLT3LG antibody PA1914. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-10
Question
I am interested in to test anti-Flt3 ligand/FLT3LG antibody PA1914 on human leukocyte for research purposes, then I may be interested in using anti-Flt3 ligand/FLT3LG antibody PA1914 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The products we sell, including anti-Flt3 ligand/FLT3LG antibody PA1914, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-16
Question
My question regarding product PA1914, anti-Flt3 ligand/FLT3LG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1914 anti-Flt3 ligand/FLT3LG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
Would anti-Flt3 ligand/FLT3LG antibody PA1914 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of leukocyte, FLT3LG is highly expressed in leukocyte. So, it is likely that anti-Flt3 ligand/FLT3LG antibody PA1914 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2019-07-25
Question
I see that the anti-Flt3 ligand/FLT3LG antibody PA1914 works with WB, what is the protocol used to produce the result images on the product page?
M. Parker
Verified customer
Asked: 2017-06-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-20
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Flt3 ligand/FLT3LG antibody PA1914. Let me know if you need anything else.
Z. Edwards
Verified customer
Asked: 2014-06-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-10
