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Product Info Summary
| SKU: | A00901-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Flk-1 KDR Antibody
View all VEGFR2/KDR/Flk-1 Antibodies
SKU/Catalog Number
A00901-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Flk-1 KDR Antibody catalog # A00901-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Flk-1 KDR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00901-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human VEGFR2. AA range:917-966
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00901-1 is reactive to KDR in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
151527 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00901-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using Flk-1 Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using Flk-1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For KDR (Source: Uniprot.org, NCBI)
Gene Name
KDR
Full Name
Vascular endothelial growth factor receptor 2
Weight
151527 MW
Superfamily
protein kinase superfamily
Alternative Names
Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD309;KDR;FLK1, VEGFR2; KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDR, check out the KDR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Flk-1 KDR Antibody (A00901-1)
Hello CJ!
A00901-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Flk-1 KDR Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Flk-1 antibody A00901-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
I was wanting to use to test anti-Flk-1 antibody A00901-1 on mouse lung for research purposes, then I may be interested in using anti-Flk-1 antibody A00901-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
The products we sell, including anti-Flk-1 antibody A00901-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-02
Question
I was wanting to use your anti-Flk-1 antibody for WB for mouse lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
It shows on the product datasheet, A00901-1 anti-Flk-1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-06
Question
We have observed staining in human lung. What should we do? Is anti-Flk-1 antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
From literature lung does express KDR. From Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2019-10-29
Question
Is this A00901-1 anti-Flk-1 antibody reactive to the isotypes of KDR?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
The immunogen of A00901-1 anti-Flk-1 antibody is Synthesized peptide derived from human Flk-1 around the non-phosphorylation site of Y951. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-24
Question
Does anti-Flk-1 antibody A00901-1 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
According to the expression profile of lung, KDR is highly expressed in lung. So, it is likely that anti-Flk-1 antibody A00901-1 will work for WB with lung.
Boster Scientific Support
Answered: 2019-07-19
Question
We are currently using anti-Flk-1 antibody A00901-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
The anti-Flk-1 antibody (A00901-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-11
Question
Our team were well pleased with the WB result of your anti-Flk-1 antibody. However we have observed positive staining in umbilical vein cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?
D. Jha
Verified customer
Asked: 2018-06-18
Answer
According to literature, umbilical vein does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454
Boster Scientific Support
Answered: 2018-06-18
Question
Is a blocking peptide available for product anti-Flk-1 antibody (A00901-1)?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
We do provide the blocking peptide for product anti-Flk-1 antibody (A00901-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-07
Question
I see that the anti-Flk-1 antibody A00901-1 works with WB, what is the protocol used to produce the result images on the product page?
J. Krishna
Verified customer
Asked: 2018-06-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-07
Question
I have a question about product A00901-1, anti-Flk-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00901-1 anti-Flk-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-29
Question
I would like using your anti-Flk-1 antibody for positive regulation of cell population proliferation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
M. Parker
Verified customer
Asked: 2017-08-16
Answer
Thank you for your inquiry. This A00901-1 anti-Flk-1 antibody is validated on hela cells. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-16
Question
Do you have a BSA free version of anti-Flk-1 antibody A00901-1 available?
M. Zhao
Verified customer
Asked: 2017-02-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Flk-1 antibody A00901-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-02-20
Question
Please see the WB image, lot number and protocol we used for lung using anti-Flk-1 antibody A00901-1. Please let me know if you require anything else.
L. Li
Verified customer
Asked: 2015-03-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-03-30
Question
Will A00901-1 anti-Flk-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Lewis
Verified customer
Asked: 2013-09-12
Answer
You can see on the product datasheet, A00901-1 anti-Flk-1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-09-12
