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Product Info Summary
| SKU: | M00399-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-FLI1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00399-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-FLI1 Rabbit Monoclonal Antibody catalog # M00399-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FLI1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00399-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ECC-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FLI1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00399-1 is reactive to FLI1 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
50982 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00399-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
Positive Control
WB: Jurkat cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of FLI1 expression in Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using FLI1 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For FLI1 (Source: Uniprot.org, NCBI)
Gene Name
FLI1
Full Name
Friend leukemia integration 1 transcription factor
Weight
50982 MW
Superfamily
ETS family
Alternative Names
Friend leukemia integration 1 transcription factor;Proto-oncogene Fli-1;Transcription factor ERGB;FLI1; FLI1 BDPLT21, EWSR2, SIC-1 Fli-1 proto-oncogene, ETS transcription factor Friend leukemia integration 1 transcription factor|Ewing sarcoma breakpoint region 2|Friend leukemia virus integration 1|transcription factor ERGB
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FLI1, check out the FLI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FLI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FLI1 Rabbit Monoclonal Antibody (M00399-1)
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No publications found for M00399-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-FLI1 Rabbit Monoclonal Antibody
Question
We are currently using anti-FLI1 Rabbit Monoclonal antibody M00399-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The anti-FLI1 Rabbit Monoclonal antibody (M00399-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-FLI1 Rabbit Monoclonal antibody M00399-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
Can you help my question with product M00399-1, anti-FLI1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00399-1 anti-FLI1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-09
Question
We purchased anti-FLI1 Rabbit Monoclonal antibody for WB on lymph last year. I am using mouse, and We are going to use the antibody for IHC next. We need examining lymph as well as placenta thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I looked at the website and datasheets of our anti-FLI1 Rabbit Monoclonal antibody and I see that M00399-1 has been tested on mouse in both WB and IHC. Thus M00399-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-13
Question
See below the WB image, lot number and protocol we used for amygdala using anti-FLI1 Rabbit Monoclonal antibody M00399-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Would anti-FLI1 Rabbit Monoclonal antibody M00399-1 work for WB with amygdala?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
According to the expression profile of amygdala, FLI1 is highly expressed in amygdala. So, it is likely that anti-FLI1 Rabbit Monoclonal antibody M00399-1 will work for WB with amygdala.
Boster Scientific Support
Answered: 2019-09-25
Question
Would M00399-1 anti-FLI1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
As indicated on the product datasheet, M00399-1 anti-FLI1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-30
Question
Is a blocking peptide available for product anti-FLI1 Rabbit Monoclonal antibody (M00399-1)?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We do provide the blocking peptide for product anti-FLI1 Rabbit Monoclonal antibody (M00399-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-27
Question
Is this M00399-1 anti-FLI1 Rabbit Monoclonal antibody reactive to the isotypes of FLI1?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The immunogen of M00399-1 anti-FLI1 Rabbit Monoclonal antibody is A synthesized peptide derived from human FLI1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-15
Question
I see that the anti-FLI1 Rabbit Monoclonal antibody M00399-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-15
Question
I was wanting to use your anti-FLI1 Rabbit Monoclonal antibody for WB for mouse amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse amygdala identification?
K. Anderson
Verified customer
Asked: 2018-10-11
Answer
As indicated on the product datasheet, M00399-1 anti-FLI1 Rabbit Monoclonal antibody has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-11
Question
We want using your anti-FLI1 Rabbit Monoclonal antibody for megakaryocyte development studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
We appreciate your inquiry. This M00399-1 anti-FLI1 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-20
Question
I am interested in to test anti-FLI1 Rabbit Monoclonal antibody M00399-1 on mouse amygdala for research purposes, then I may be interested in using anti-FLI1 Rabbit Monoclonal antibody M00399-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
The products we sell, including anti-FLI1 Rabbit Monoclonal antibody M00399-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-08
Question
Do you have a BSA free version of anti-FLI1 Rabbit Monoclonal antibody M00399-1 available?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-FLI1 Rabbit Monoclonal antibody M00399-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-23
Question
Our team were happy with the WB result of your anti-FLI1 Rabbit Monoclonal antibody. However we have seen positive staining in bone marrow nucleus. using this antibody. Is that expected? Could you tell me where is FLI1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
Based on literature, bone marrow does express FLI1. Generally FLI1 expresses in nucleus. Regarding which tissues have FLI1 expression, here are a few articles citing expression in various tissues:
Amygdala, Placenta, and Thymus, Pubmed ID: 14702039
Blood, Pubmed ID: 8439553
Bone marrow, Pubmed ID: 1522903
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 7542907
Boster Scientific Support
Answered: 2017-10-31
Question
We have been able to see staining in human blood. Do you have any suggestions? Is anti-FLI1 Rabbit Monoclonal antibody supposed to stain blood positively?
A. Jones
Verified customer
Asked: 2015-03-02
Answer
From what I have seen in literature blood does express FLI1. From what I have seen in Uniprot.org, FLI1 is expressed in leukocyte, bone marrow, blood, amygdala, placenta thymus, lymph, placenta, leukemic t-cell, among other tissues. Regarding which tissues have FLI1 expression, here are a few articles citing expression in various tissues:
Amygdala, Placenta, and Thymus, Pubmed ID: 14702039
Blood, Pubmed ID: 8439553
Bone marrow, Pubmed ID: 1522903
Leukemic T-cell, Pubmed ID: 19690332
Lymph, Pubmed ID: 15489334
Placenta, Pubmed ID: 7542907
Boster Scientific Support
Answered: 2015-03-02
