Anti-FLI1 (Ewing s Sarcoma & Endothelial Marker) Monoclonal Antibody

Product Name

Anti-FLI1 (Ewing s Sarcoma & Endothelial Marker) Monoclonal Antibody

View all FLI1 Antibodies

SKU/Catalog Number

M00399

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-FLI1 (Ewing s Sarcoma & Endothelial Marker) Monoclonal Antibody (Catalog # M00399). Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-FLI1 (Ewing s Sarcoma & Endothelial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00399)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: FLI1/1312

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (aa 107-261) of human FLI1 protein with hexa-histidine tag

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00399 is reactive to FLI1 in Human

Applications

M00399 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

50982 MW

Background of FLI1

Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing's Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Western Blot of THP1 and Raji cell lysate using Anti-FLI1 Monoclonal Antibody (FLI1/1312)

Gene/Protein Information For FLI1 (Source: Uniprot.org, NCBI)

Alternative Names

ERGB; EWSR2; FLI1; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC-1; Transcription factor ERGB FLI1 BDPLT21, EWSR2, SIC-1 Fli-1 proto-oncogene, ETS transcription factor Friend leukemia integration 1 transcription factor|Ewing sarcoma breakpoint region 2|Friend leukemia virus integration 1|transcription factor ERGB

For more info on FLI1, check out the FLI1 Infographic

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In this infographic, you will see the following information for FLI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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