Anti-Filamin A/FLNA Antibody Picoband®

Filamin A antibody

Boster Bio Anti-Filamin A/FLNA Antibody Picoband® catalog # A00502-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00502-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-Filamin A/FLNA Antibody Picoband®

View all Filamin A Antibodies

SKU/Catalog Number

A00502-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Filamin A/FLNA Antibody Picoband® catalog # A00502-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Filamin A/FLNA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00502-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Filamin A/FLNA recombinant protein (Position: E451-Q638).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00502-1 is reactive to FLNA in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

281 kDa

Calculated molecular weight

44565 MW

Background of Filamin A

Filamin A, alpha  (FLNA) is a protein that in humans is encoded by the FLNA gene. It is mapped to Xq28. The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00502-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: human A549 whole cell, human U2OS whole cell, human Hela whole cell, human PC-3 whole cell, rat ovarian tissue, mouse lung tissue, mouse HEPA1-6 whole cell
IHC: human Lung cancer tissue, human Lung cancer tissue, human skeletal muscle tissue, mouse intestine tissue, rat intestine tissue, rat intestine tissue
ICC/IF: A549 cell
FCM: U87 cell

Validation Images & Assay Conditions

Gene/Protein Information For FLNA (Source: Uniprot.org, NCBI)

Gene Name

FLNA

Full Name

Filamin-A

Weight

44565 MW

Superfamily

filamin family

Alternative Names

Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin; FLNA; FLN, FLN1 FLNA ABP-280, ABPX, CSBS, CVD1, FGS2, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD filamin A filamin-A|actin binding protein 280|alpha-filamin|endothelial actin-binding protein|epididymis secretory sperm binding protein|filamin A, alpha|filamin-1|non-muscle filamin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FLNA, check out the FLNA Infographic

FLNA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FLNA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A00502-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Filamin A/FLNA Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-Filamin A/FLNA Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-Filamin A/FLNA Antibody Picoband®

Question

What is the dilutent to add to A00502-1 antibody reconstitution for IHC? Can distilled water be used or not?

Verified customer

Asked: 2019-09-19

Answer

Yes, you can reconstitute the Anti-Filamin A/FLNA Antibody Picoband A00502-1 by adding 0.2ml of distilled water which will yield a concentration of 500ug/ml, and then use PBS for serial dilution.

Boster Scientific Support

Answered: 2019-09-20

Question

How many vials do you suggest in IHC-P for 100 slides application using A00502-1 antibody?

Verified customer

Asked: 2019-07-24

Answer

The suggested working concentration for IHC-P is 1ug/ml. Our lab technicians usually add 50ul onto each slide. It takes 60 slides and 5ug of Anti-Filamin A/FLNA Antibody Picoband A00502-1 for 100 slides.

Boster Scientific Support

Answered: 2019-07-26

Question

Anti-Filamin A/FLNA Picoband

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

The suggested working concentration for IHC-P is 1ug/ml. Our lab technicians usually add 50ul onto each slide. It takes 3ug of M00334-6 for 60 slides and 5ug of A00502-1 for 100 slides.

Boster Scientific Support

Answered: 2019-06-20

Question

We are currently using anti-Filamin A/FLNA antibody A00502-1 for rat tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

The anti-Filamin A/FLNA antibody (A00502-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-15

Order DetailsPrice
A00502-1

100μg

$370
A00502-1-10ug

10μg sample (liquid)

$99
A00502-1-Biotin

100 μg Biotin conjugated

$570
A00502-1-Cy3

100 μg Cy3 conjugated

$570
A00502-1-Dylight488

100 μg Dylight488 conjugated

$570
A00502-1-Dylight550

100 μg Dylight550 conjugated

$570
A00502-1-Dylight594

100 μg Dylight594 conjugated

$570
A00502-1-FITC

100 μg FITC conjugated

$570
A00502-1-HRP

100 μg HRP conjugated

$570
A00502-1-APC

100 μg APC conjugated

$670
A00502-1-PE

100 μg PE conjugated

$670
A00502-1-iFluor647

100 μg iFluor647 conjugated

$670
A00502-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A00502-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.