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Key=description, value=The Filamin A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Filamin A protein expression profile in cells. The kit can be used for measuring the relative amounts of Filamin A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Filamin A.
Key=short_description, value=The Filamin A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Filamin A protein expression profile in cells. The kit can be used for measuring the relative amounts of Filamin A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Filamin A.
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Key=synonyms, value=ABP-280;Alpha-filamin;Endothelial actin-binding protein;FLN;FLN1;Filamin 1;Nonmuscle filamin;filamin A
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Key=kit_components, value=Filamin A
Key=subcellular_localization, value=Cytoplasm, cell cortex. Cytoplasm, cytoskeleton.
Key=tissue_specificity, value=Ubiquitous.
Key=protein_function, value= Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface- localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (By similarity).
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Filamin A Colorimetric Cell-Based ELISA Kit

Filamin A ELISA kit, cell-based

The Filamin A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Filamin A protein expression profile in cells. The kit can be used for measuring the relative amounts of Filamin A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Filamin A.

Product Info Summary

SKU: EKC1216
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

Filamin A Colorimetric Cell-Based ELISA Kit

See all Filamin A products

SKU/Catalog Number

EKC1216

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Filamin A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Filamin A protein expression profile in cells. The kit can be used for measuring the relative amounts of Filamin A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Filamin A.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Filamin A Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1216)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1216 is reactive to FLNA in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1216 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Filamin A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For FLNA (Source: Uniprot.Org, NCBI)

Gene Name

FLNA

Full Name

Filamin-A

Weight

280739 MW

Superfamily

filamin family

Alternative Names

ABP-280;Alpha-filamin;Endothelial actin-binding protein;FLN;FLN1;Filamin 1;Nonmuscle filamin;filamin A FLNA ABP-280, ABPX, CSBS, CVD1, FGS2, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD filamin A filamin-A|actin binding protein 280|alpha-filamin|endothelial actin-binding protein|epididymis secretory sperm binding protein|filamin A, alpha|filamin-1|non-muscle filamin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FLNA, check out the FLNA Infographic

FLNA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FLNA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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