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Product Info Summary
| SKU: | PB10061 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-FGF19 Antibody Picoband®
SKU/Catalog Number
PB10061
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FGF19 Antibody Picoband® catalog # PB10061. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FGF19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10061)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FGF19 recombinant protein (Position: L25-K216).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10061 is reactive to FGF19 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
24003 MW
Background of FGF-19
FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity. FGF19 is the orthologous protein in humans. They are often referred together as FGF15/19.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10061 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA(Cap) , 1-5μg/ml, Human, -
Positive Control
WB: HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FGF19 using anti-FGF19 antibody (PB10061).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (Catalog # PB10061) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.
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Figure 2. Sandwich ELISA - Recombinant human fgf19 protein standard curve.
Use in combination with reagents from Human fgf19 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1160).
Protein Target Info & Infographic
Gene/Protein Information For FGF19 (Source: Uniprot.org, NCBI)
Gene Name
FGF19
Full Name
Fibroblast growth factor 19
Weight
24003 MW
Superfamily
heparin-binding growth factors family
Alternative Names
Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533; FGF19 fibroblast growth factor 19 fibroblast growth factor 19|FGF-19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FGF19, check out the FGF19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FGF19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FGF19 Antibody Picoband® (PB10061)
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Customer Q&As
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3 Customer Q&As for Anti-FGF19 Antibody Picoband®
Question
Does PB10061 anti-FGF19 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
You can see on the product datasheet, PB10061 anti-FGF19 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-20
Question
Do you have a BSA free version of anti-FGF19 antibody PB10061 available?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF19 antibody PB10061 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-30
Question
Is this PB10061 anti-FGF19 antibody reactive to the isotypes of FGF19?
L. Jones
Verified customer
Asked: 2018-12-11
Answer
The immunogen of PB10061 anti-FGF19 antibody is E. coli-derived human FGF19 recombinant protein (Position: L25-K216). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-11
