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Product Info Summary
| SKU: | PB10062 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Fgf19(Fgf15) Antibody
View all Fgf19(Fgf15) Antibodies
SKU/Catalog Number
PB10062
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fgf19(Fgf15) Antibody catalog # PB10062. Tested in IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fgf19(Fgf15) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10062)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10062 is reactive to Fgf19(Fgf15) in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
24003 MW
Background of Fgf19(Fgf15)
FGF15/19 refers to two orthologous fibroblast growth factors which share 50% aminoacid identity and have similar functions. FGF15 was described in the mouse; FGF19 was found in humans and other species. They share physiological functions and so are often referred to as FGF15/19 or as FGF15/FGF19. They were first described in developing fetal brain. They are now known to be produced in the ileum, and under certain circumstances in the liver and biliary tree. It is thought their principal function is in response to bile acid absorption occurring after meals. FGF15 and FGF19 have similar roles in regulating bile acid synthesis and also glucose metabolism in the liver.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10062 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Positive Control
IHC: mouse colon tissue, rat colon tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Fgf19(Fgf15) using anti-Fgf19(Fgf15) antibody (PB10062).
Fgf19(Fgf15) was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Fgf19(Fgf15) using anti-Fgf19(Fgf15) antibody (PB10062).
Fgf19(Fgf15) was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Fgf19(Fgf15) (Source: Uniprot.org, NCBI)
Gene Name
Fgf19(Fgf15)
Full Name
Weight
24003 MW
Alternative Names
Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fgf19(Fgf15), check out the Fgf19(Fgf15) Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fgf19(Fgf15): database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fgf19(Fgf15) Antibody (PB10062)
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Customer Q&As
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2 Customer Q&As for Anti-Fgf19(Fgf15) Antibody
Question
Should ‘Mouse’ be added to species reactivity for PB10062?
Verified customer
Asked: 2022-04-08
Answer
Yes, Anti-Fgf19(Fgf15) Antibody Picoband™ (PB10062) reacts with Mouse and Rat.
Boster Scientific Support
Answered: 2022-04-08
Question
We are currently using anti-FGF19 antibody PB10062 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The anti-FGF19 antibody (PB10062) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-13
