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Product Info Summary
| SKU: | PB9360 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-RBP4 Antibody Picoband®
View all RBP4/Retinol-Binding Protein 4 Antibodies
SKU/Catalog Number
PB9360
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RBP4 Antibody Picoband® catalog # PB9360. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RBP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9360)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RBP4 recombinant protein (Position: E19-201L). Human RBP4 shares 86% amino acid (aa) sequence identity with both mouse and rat RBP4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9360 is reactive to RBP4 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23010 MW
Background of RBP4/Retinol-Binding Protein 4
RBP4 is also known as RDCCAS. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9360 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: Rat Liver Tissue, Mouse Liver Tissue, HEPG2 Whole Cell, COLO320 Whole Cell
IHC: human liver cancer tissue, human liver cancer tissue, mouse kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Anti-RBP4 Picoband antibody, PB9360, Western blotting
All lanes: Anti RBP4 (PB9360) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: HEPG2 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
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Figure 2. IHC analysis of RBP4 using anti-RBP4 antibody (PB9360).
RBP4 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of RBP4 using anti-RBP4 antibody (PB9360).
RBP4 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of RBP4 using anti-RBP4 antibody (PB9360).
RBP4 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of RBP4 using anti-RBP4 antibody (PB9360).
RBP4 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RBP4 (Source: Uniprot.org, NCBI)
Gene Name
RBP4
Full Name
Retinol-binding protein 4
Weight
23010 MW
Superfamily
calycin superfamily
Alternative Names
Retinol-binding protein 4;Plasma retinol-binding protein;PRBP;RBP;Plasma retinol-binding protein (1-182);Plasma retinol-binding protein (1-181);Plasma retinol-binding protein (1-179);Plasma retinol-binding protein (1-176);RBP4;PRO2222; RBP4 MCOPCB10, RDCCAS retinol binding protein 4 retinol-binding protein 4|PRBP|RBP|plasma retinol-binding protein|retinol binding protein 4, plasma|retinol-binding protein 4, interstitial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RBP4, check out the RBP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RBP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RBP4 Antibody Picoband® (PB9360)
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1 Customer Q&As for Anti-RBP4 Antibody Picoband®
Question
We are currently using anti-RBP4 antibody PB9360 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
V. Krishna
Verified customer
Asked: 2014-12-15
Answer
The anti-RBP4 antibody (PB9360) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-15
