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Key=description, value=The FER Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FER protein expression profile in cells. The kit can be used for measuring the relative amounts of FER in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FER.
Key=short_description, value=The FER Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FER protein expression profile in cells. The kit can be used for measuring the relative amounts of FER in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FER.
Key=gene_name, value=FER
Key=synonyms, value=p94-FER;c-FER;Tyrosine kinase 3
Key=sample_type, value=Cell lines
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Key=subcellular_localization, value=Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein;Cytoplasmic side. Cell projection. Cell junction. Membrane; Peripheral membrane protein;Cytoplasmic side. Nucleus. Cytoplasm, cell cortex. Associated with the chromatin. Detected on microtubules in polarized and motile vascular endothelial cells. Colocalizes with F-actin at the cell cortex. Colocalizes with PECAM1 and CTNND1 at nascent cell- cell contacts.
Key=tissue_specificity, value=Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed.
Key=protein_function, value= Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.
Key=storage, value=Store at 4°C for up to 6 months.
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FER Colorimetric Cell-Based ELISA Kit

FER ELISA kit, cell-based

The FER Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FER protein expression profile in cells. The kit can be used for measuring the relative amounts of FER in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FER.

Product Info Summary

SKU: EKC1210
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA
Sample Types: Cell lines

Product Name

FER Colorimetric Cell-Based ELISA Kit

See all FER products

SKU/Catalog Number

EKC1210

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The FER Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor FER protein expression profile in cells. The kit can be used for measuring the relative amounts of FER in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on FER.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

FER Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1210)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1210 is reactive to FER in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1210 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-FER Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For FER (Source: Uniprot.Org, NCBI)

Gene Name

FER

Full Name

Tyrosine-protein kinase Fer

Weight

94638 MW

Superfamily

protein kinase superfamily

Alternative Names

p94-FER;c-FER;Tyrosine kinase 3 FER PPP1R74, TYK3, p94-Fer FER tyrosine kinase tyrosine-protein kinase Fer|feline encephalitis virus-related kinase FER|fer (fps/fes related) tyrosine kinase|fujinami poultry sarcoma/Feline sarcoma-related protein Fer|phosphoprotein NCP94|protein phosphatase 1, regulatory subunit 74|proto-oncogene c-Fer|tyrosine kinase 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FER, check out the FER Infographic

FER infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FER: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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