Product Info Summary
SKU: | A08695 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-FBP3 FUBP3 Antibody
SKU/Catalog Number
A08695
Size
100μl
Form
Liquid
Description
Boster Bio Anti-FBP3 FUBP3 Antibody catalog # A08695. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FBP3 FUBP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08695)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human FBP3. at AA rangle: 180-260
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08695 is reactive to FUBP3 in Human, Mouse, Rat
Applications
A08695 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
61640 MW
Background of FUBP3
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
![a08695 western blotting analysis 1 a08695 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a08695-western-blotting-analysis-1.jpg)
Click image to see more details
Western Blot (WB) analysis of specific cells using FBP3 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For FUBP3 (Source: Uniprot.org, NCBI)
Gene Name
FUBP3
Full Name
Far upstream element-binding protein 3
Weight
61640 MW
Alternative Names
far upstream element (FUSE) binding protein 3; far upstream element-binding protein 3; FBP3; FLJ25229; FUSE-binding protein 3 FUBP3 FBP3 far upstream element binding protein 3 far upstream element-binding protein 3|FUSE-binding protein 3|far upstream element (FUSE) binding protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FUBP3, check out the FUBP3 Infographic
![FUBP3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FUBP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FBP3 FUBP3 Antibody (A08695)
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No publications found for A08695
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-FBP3 FUBP3 Antibody
Question
Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-FBP3 antibody A08695. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-29
Question
We are interested in to test anti-FBP3 antibody A08695 on rat leukemic t-cell for research purposes, then I may be interested in using anti-FBP3 antibody A08695 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Lewis
Verified customer
Asked: 2019-12-24
Answer
The products we sell, including anti-FBP3 antibody A08695, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-24
Question
Is this A08695 anti-FBP3 antibody reactive to the isotypes of FUBP3?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
The immunogen of A08695 anti-FBP3 antibody is Synthesized peptide derived from the Internal region of human FBP3. at AA rangle: 180-260. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-27
Question
Does A08695 anti-FBP3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
As indicated on the product datasheet, A08695 anti-FBP3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-23
Question
We are currently using anti-FBP3 antibody A08695 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
J. Rodriguez
Verified customer
Asked: 2017-02-14
Answer
The anti-FBP3 antibody (A08695) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-14
Question
I see that the anti-FBP3 antibody A08695 works with WB, what is the protocol used to produce the result images on the product page?
K. Banerjee
Verified customer
Asked: 2013-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-16
Question
Will anti-FBP3 antibody A08695 work for WB with leukemic t-cell?
T. Walker
Verified customer
Asked: 2013-08-12
Answer
According to the expression profile of leukemic t-cell, FUBP3 is highly expressed in leukemic t-cell. So, it is likely that anti-FBP3 antibody A08695 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2013-08-12